葡萄原花青素对乙醇诱发小鼠肝细胞增殖活性损伤的防护作用

目的 研究葡萄原花青素(GPC)对乙醇诱发小鼠肝细胞增殖活性损伤的防护作用.方法 将小鼠随机分为5组,乙醇对照组和各剂量GPC组小鼠均每天经口灌胃乙醇4 g/kg体质量,低、中、高剂量GPC组同时分别给予GPC 50、100和200mg/kg体质量,连续4周.眼球取血,颈椎脱臼处死小鼠,取肝组织用MTT法检测各组小鼠的...

THE PROTECTIVE EFFECT OF GRAPE PROCYANIDINS ON THE PROLIFERATIVE ACTIVITY OF MICE HEPATOCYTES INJURED BY ETHANOL

Objective To study the protective effect of grape procyanidins(GPC) on the proliferative activity of mouse hepatocytes damaged by ethanol. Methods The mice were randomly divided into five groups,Those in low-,moderate-and high-dose GPC groups were intragastrically given 50,100 and 200 mg/kg of GPC,and those in ethanol group,4 g/kg of ethanol were given,respectively,for four weeks.All the mice were then sacrificed.The liver cell multiplication activity was determined by MTT assay,the pathology of liver tissue section stained by HE was observed light-microscopically;the expressions of Bcl-2 and Bax protein in liver cells were measured by immunohistochemistry method;the serum alanine aminotransferase(ALT) was detected by automatic biochemical analyzer,and the serum TNF-α was detected by ELISA method. Results The ALT levels of high-dose GPC group were lower than that of the ethanol group(F=14.05;q=4.29,5.53;P<0.05),and the cell proliferation activity was higher(F=68.68;q=5.71,8.57;P<0.05).Compared with the ethanol group,the ratio of Bcl-2/Bax was significantly higher in the high-dose GPC group(F=388.03;q=4.90;P<0.05),the TNF-α levels of the high-and moderate-dose GPC groups were lower(F=10.09;q=3.01,5.68;P<0.05).GPC improved the liver injury in different degrees. Conclusion GPC can prevent alcohol-induced injury of liver cell multiplication activity by regulating the ratio of Bcl-2/Bax and lowering serum TNF-α concentration.