| mtLSU-巢式PCR检测实验大鼠卡氏肺孢子虫的实验研究 |
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| 引用本文： | 张小娟,杨芳芳,欧阳颐,王鸽,黎学铭,林睿. mtLSU-巢式PCR检测实验大鼠卡氏肺孢子虫的实验研究[J]. 热带医学杂志, 2011, 11(4): 372-374,432 |
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| 作者姓名： | 张小娟 杨芳芳 欧阳颐 王鸽 黎学铭 林睿 |
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| 作者单位： | 1. 广西医科大学寄生虫教研室,广西,南宁,530021
2. 广西疾病预防控制中心,广西,南宁,530028 |
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| 基金项目： | 广西人事厅留学回国人员基金项目，广西科技厅重点项目，广西科技厅科技攻关项目 |
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| 摘 要： | 目的 对mt1SU-巢式PCR方法 检测大鼠卡氏肺孢子虫的应用价值以及基因序列进行评价.方法 采用地塞米松免疫抑制法诱导大鼠感染肺孢子虫;实验组10只,对照组1只;诱导至第7周时收集实验组及对照组大鼠肺组织和支气管肺泡灌洗液(BALF)标本,采用 mtLSU-巢式PCR方法 对人源与鼠源肺抱子虫共有的基因进行扩增和序列...
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| 关 键 词： | 卡氏肺孢子虫 线粒体核糖体大亚基RNA基因(mtLSU) 巢式PCR 基因序列 |
mtLSU-nested polymerase chain reaction in the detection of Pheumocystis carinii in experimental rat |
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| ZHANG Xiao-juan,YANG Fang-fang,OU Yang-yi,WANG Ge,LI Xue-ming,LIN Rui. mtLSU-nested polymerase chain reaction in the detection of Pheumocystis carinii in experimental rat[J]. Journal Of Tropical Medicine, 2011, 11(4): 372-374,432 |
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| Authors: | ZHANG Xiao-juan YANG Fang-fang OU Yang-yi WANG Ge LI Xue-ming LIN Rui |
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| Affiliation: | ZHANG Xiao-juan,YANG Fang-fang,OU Yang-yi,WANG Ge,LI Xue-ming,LIN Rui(Department of Parasitology,Guangxi Medical University,Guangxi,Nanning 530021,China) |
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| Abstract: | Objective To investigate the sensitivity and diagnostic value of nested PCR with mtLSU in the detection of Pneumocystis carinii(P.carinii) in rat and to determine the mtLSU gene sequence of P.carinii from Wiatar rat.Methods P.carinii infection model was established by subcutaneous injection of an immunodepressant dexamethasone in rats.The study was carried out in 2 groups,including a control group(1 rat) and an experimental group(10 rats).Bronchoalveolar lavage fluid(BALF) and lung tissue from the rats were... |
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| Keywords: | Pheumocystis carinii mt-LSU nested PCR gene sequence |
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