鼻息肉中 IL-15 mRNA的表达及其作用

目的：观察IL-15mRNA在鼻息肉与下鼻甲中表达的差异及其与Bcl-2、Bcl-XL表达的关系，明确鼻息肉中IL-15的来源，探讨其在鼻息肉发病机制中的作用。方法用原位杂交的方法测定24例鼻息肉与8例下鼻甲的IL-15mRNA的表达情况，同时用免疫组化法检测其组织中Bcl-2及Bcl-XL的表达情况。结果鼻息肉组中IL-15mRNA在黏膜下固有层浸润的以EOS为主的炎症细胞有很强的表达，与下鼻甲组IL-15mRNA的表达差异有统计学意义（P＜0．05）。 IL-15mRNA在多数鼻息肉中的黏膜下腺体上皮细胞有表达，且强于在下鼻甲组的表达。黏膜下固有层炎症细胞IL-15mRNA的表达与Bcl-XL的表达有相关性（ r＝0．592）。结论 IL-15通过旁分泌或自分泌的方式作用于位于黏膜下固有层中以嗜酸性粒细胞为主的炎症细胞，并通过Bcl-XL途径诱导的凋亡抑制在鼻息肉的发病中可能起重要作用。 IL-15在鼻息肉中黏膜下腺体的表达强于在下鼻甲中的表达，可能参与了鼻息肉形成过程中黏膜下腺体分泌功能紊乱的病理过程。

Expression and significance of IL-15mRNA in nasal polyps

Objective To investigate the expression of IL-15mRNA in nasal polyps and its relation with Bcl-2 and Bcl-XL and to find out the source of IL-15 in nasal polyps and its role in nasal polyps.Methods The in situ hybridization （ISH） method was used to detect the expression of IL-15mRNA in nasal polyps and inferior nasal concha.Immunohis-tochemistry was used to detect the expressions of Bcl-2 and Bcl-XL in nasal polyps and inferior nasal concha.Results The expression of IL-15mRNA in nasal polyps was higher than that in inferior nasal concha （P〈0.05）, so did the Bcl-XL expression（P〈0.01）.The expression of Bcl-2 was not significant between the nasal polyps and the inferior nasal concha.The expressions of IL-15mRNA and Bcl-XL were positively correlated with each other.Conclusion IL-15 may play an important role in the pathogenesis of nasal polyps by inhibiting the apoptosis of eosinophlis and other inflammatory cells, which maybe do by up regulating the expression of Bcl-XL by the way of autocrine or paracrine.