内蒙古地区炭疽芽胞杆菌基因单核苷酸多态性研究

目的研究内蒙古地区炭疽芽胞杆菌基因单核苷酸多态性(SNP)特征。方法选择内蒙古地区不同分离年代、地点和来源的22株炭疽芽胞杆菌,采用实时荧光定量聚合酶链反应(real-time PCR)方法扩增染色体上的13个SNP位点,进行聚类分析。结果使用的13个SNP位点中8个位点相同,5个位点存在多态性。通过聚类分析,22株炭疽芽胞杆菌可分为4个群,属于A.Br.Aust94亚群和A.Br.008/009亚群的菌株各有1株,A.Br.Ames亚群和A.Br.001/002亚群菌株占大多数。结论内蒙古地区炭疽芽胞杆菌基因SNP位点具有遗传稳定性,目前5个SNP位点可作为内蒙古地区炭疽杆菌荚膜质粒SNP位点基因分型的指标,A.Br.Ames亚群和A.Br.001/002亚群是内蒙古地区优势亚群。

Study on the single nucleotide polymorphism in gene of Bacillus anthracis isolated in Inner Mongolia

Objective To study the characteristics of single nucleotide polymorphism（ SNP） in gene of Bacillus anthracis isolated in Inner Mongolia. Methods Twenty two Bacillus anthracis isolates from different sources were selected. The 13 SNP sites were amplified by using real-time PCR,and related clustering analysis was conducted.Results The identified isolates had the same sequence in 8 sites and different nucleotide sequence in the other 5 sites of the 13 SNP sites. The 22 Bacillus anthracis isolates were divided into 4 groups by cluster analysis. One A. Br. Aust94strain and 1 A. Br. 008 /009 strain were detected. Twenty strains belonged to A. Br. Ames and A. Br. 001 /002 subgroups.Conclusion The analysis showed that single nucleotide genetic stability and the 5 discriminating SNP sites could be used as indicators in genotyping the Bacillus anthracis isolated in Inner Mongolia. A. Br. Ames and A. Br. 001 /002 subgroups were the clusters predominating in Inner Mongolia.