变应性鼻炎鼻粘膜P物质受体的研究

目的探讨Ｐ物质受体（ｓｕｂｓｔａｎｃｅＰｒｅｃｅｐｔｏｒ，ＳＰＲ）在变应性鼻炎发病中的作用。方法选健康大鼠２０只，雌雄不拘，随机分为实验组和对照组各１０只。实验组用卵清蛋白腹腔注射免疫，继之鼻局部免疫建立大鼠变应性鼻炎模型，取该模型和健康对照组动物的鼻粘膜行常规ＨＥ染色及免疫组化染色，以观察ＳＰＲ在鼻粘膜中的分布、密度及其在该模型中的动态变化。结果ＳＰＲ广泛分布于健康鼻粘膜上皮细胞、血管内皮细胞、腺细胞及导管、肥大细胞、嗜酸粒细胞、浆细胞和其它单核细胞。变应性鼻炎鼻粘膜中上述部位ＳＰＲ染色加深，阳性颗粒增多，密度增强，同时ＳＰＲ阳性细胞数量增多。结论变应性鼻炎鼻粘膜中ＳＰＲ表达增多，提示ＳＰＲ在变应性鼻炎发病中发挥关键作用

Distribution of substance P receptors in rat nasal mucosa of allergic rhinitis

Objective To explore the potential role of substance P receptor (SPR) in the initiation and development of allergic rhinitis (AR). Methods Twenty healthy rats were randomly divided into two groups. A model of allergic rhinitis in rat was established by using ovalbumin intraperitoneal immunization and nasal antigen challenge. The nasal mucosa obtained from seven out of ten AR models as well as ten normal controls were studied routinely by HE and immunohistochemical staining to observe the distribution and changes of SPR. Results The study showed that immunoreactivity to SPR was present both on the surface and in the cytoplasm of epithelial cells, eosinophils, glandular cells and its ducts, mast cells, plasmocyte and other monocytes. During nasal hypersensiti veness, the staining density and the positive staining cells increased significantly as compared with those in normal controls. Conclusion The increase of SPR in nasal mucosa in rat AR model indicates that SPR might play a critical role in the development of AR.