三七皂甙对氧化损伤大鼠脑能量代谢及线粒体呼吸功能的影响

目的　探讨三七皂甙对氧化损伤型类阿尔茨海默病大鼠脑组织能量代谢的影响。方法　采用二羟延胡索酸 (DHF)和FeCl3 ADP左侧脑室注射法制作大鼠氧化损伤型类AD大鼠模型 ;用高效液相法测定脑组织中含高能磷酸键的ATP、磷酸肌酸等的含量 ,用氧电极法测量线粒体呼吸链呼吸功能 ,观察分析各指标在同样插管但注射生理盐水的手术对照组以及模型组、给药组 (给三七皂甙 )之间的差异。结果　给药组ATP、磷酸肌酸显著高于模型组 ,但仍低于对照组 ,给药组ADP、AMP显著低于模型组。给药组线粒体 3、4态呼吸以及P/O显著高于模型组。结论　三七皂甙可以有效地改善氧化损伤型类AD大鼠脑能量代谢及线粒体功能 ,提示其可用于AD的治疗。

Effects of saponins of Panax Notoginseng on energy metabolism and mitochondrial respiratory function of brain in oxidative damaged rats

Objective To observe the effects of saponins of Panax notoginseng (PNS) on the brain energy metabolism and mitochondrial respiratory function of oxidative damaged rat model mimic to Alzheimer disease (AD). Methods Oxidative damaged rat model mimic AD (AD group) was established by injection of dihydroxy fumaric acid (DHF) and FeCl3 ADP into the left lateral ventricle of rats. The contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), and phosphate creatine (Pcr) in brain were determined by high performance liquid chromatography (HPLC). The mitochondrial functions (R3, R4, P/O, and OPR) were observed by Clark oxygen electrode method. The difference in the above parameters in rats in the control group (injection of saline), model group, and administration group (injection of PNS) was observed. Results The levels of ATP, Pcr, and R3, R4, P/O, and OPR of rats in AD group were significantly lower than those in the control group (0.9% NaCl instead of DHF FeCl3 ADP), but those in PNS group were higher than those in the AD group. Conclusion PNS can improve brain energy metabolism and mitochondrial function of oxidative damaged rats, suggesting that PNS could be used for the treatment of AD.