Neurokinin-1受体介导P物质对破骨细胞骨吸收功能的影响

目的 　了解P物质对体外培养大鼠破骨细胞骨吸收功能的影响和作用机制，探讨P物质在正畸牙槽骨改建中的调控机制。方法　 通过机械分离新生大鼠四肢长骨方法体外培养破骨细胞，细胞免疫组织化学染色观察NK1受体在大鼠破骨细胞内的表达情况；加入含不同浓度P物质（10-7-10-4 mol/L）和10-5 mol/L P物质受体拮抗剂（NK1受体拮抗剂）的培养液，观察P物质和NK1受体拮抗剂对破骨细胞骨吸收功能的影响。结果 免疫组化染色发现，大
鼠破骨细胞内NK1受体呈强阳性表达，着色位于细胞浆，胞核呈阴性；P物质明显增加破骨细胞的骨吸收陷窝面积（P<0.05），但未见骨吸收陷窝数目明显增加（P>0.05）。NK1受体拮抗剂抑制了P物质对破骨细胞骨吸收功能的刺激
效应。结论 NK1受体可能介导P物质增强破骨细胞骨吸收功能，从而在正畸牙槽骨改建中发挥局部调节作用。

Substance P Regulates Function of Osteoclasts via Neurokinin-1 Receptor

Objective To investigate the effects of substance P on cultured rat osteoclasts. Methods Neurokinin- 1( NK1) receptor expression in osteoclasts was examined by immunohitochemical method, and changes of bone resorption activity caused by substance P and NK1 receptor antagonists were detected by pit formation assay. Results Immunoreactivity for NK1 receptor was distributed in the cytoplasm of osteoclasts. The average of pit formation areas significantly increased with addition of substance P( 10-7- 10-4 mol/L)( P<0.05), but the number of pitformations did not change( P>0.05). NK1 receptor antagonists inhibited the enhancement of the bone resorption by substance P addition. Conclusion The findings suggested that substance P may stimulate osteoclasts and result in bone resorption by the mediation of NK1 receptor.