2004年度我院细菌检测结果及耐药性分析

目的监测我院2004年临床可疑细菌感染标本的分离培养及细菌耐药状况。方法对我院从2004年1月19日—2004年12月31日分离的235株细菌,按常规操作规程进行,必要时应用BD公司的BBLCRASTAL鉴定系统。药敏试验采用WHO推荐的K-B纸片扩散法测定细菌对药物的敏感性。结果培养率最高的细菌为大肠埃希菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌、白色假丝酵母菌等。碳青霉烯类是革兰阴性杆菌(除嗜麦芽窄食单胞菌)抗菌作用最强的抗生素。头孢哌酮/舒巴坦、头孢他啶、左氧氟沙星对革兰阴性杆菌也有很好的抗菌活性。但仍有40%~55%的大肠埃希菌对氟喹诺酮类耐药。结论头孢哌酮/舒巴坦、左氧氟沙星、环丙沙星、庆大霉素和妥布霉素对非发酵阴性杆菌的抗菌谱较广,抗菌作用也较好,另外一些耐药菌株的出现应引起我们注意。

Bacterial Isolation and Resistance Surveillance in the Year of 2004

Objective To determine bacte rial isolation and the resistance against drugs in our hospital. Methods From January 19 to December 31, 2004, a total of 235 s trains of bacteria isolated from our hospital were collected. According to norma l operational procedure, the isolates were identified by BBLCRYSTAL system of B. D Company. Susceptibility tests were conducted using an international standard m ethod. Results The most common pathogens were Escherichia colia, Staph ylococcus aureas, Pseudomonas aeruginosa, Klebsislla pneumoniae and Candida albi cans. Carbapenems were the most active antibiotics tested against Gram- negative bacilli (except stenotrophomonas maltophilia). Cefoperazone/sulbactam, ceftazidime, levofloxacin also showed excellent activity. But 40%~55% of Escheri chia coli were still resistant to fluoroquionlone. Conclusion Cefoperazone/sulbactam, levofloxacin, ciprofloxacin, gentamicinand tobramycin were most interesting antimicrobial agents with broad -spectrum. Also, bacterial resistance against drugs ought to be noticed.