山羊颞下颌关节盘细胞体外培养研究

目的:研究山羊颞下颌关节盘纤维软骨细胞体外培养的生物学特性及其损伤修复的细胞学基础.方法:切取1 月龄山羊颞下颌关节盘, II型胶原酶消化获得关节盘纤维软骨细胞.逐日观察细胞的形态变化, 测定其生长曲线,甲苯胺蓝染色、I型胶原免疫组化染色鉴定.透射电镜观察细胞超微结构.结果:原代培养的关节盘纤维软骨细胞以长梭形为主, 部分多角形, 7 d即可传代.甲苯胺蓝染色阳性, I型胶原免疫组化染色胞质内可见棕黄色颗粒.超微结构显示细胞分为2 种,软骨细胞样细胞和成纤维细胞样细胞;线粒体、内质网发达.结论:体外分离培养的山羊颞下颌关节盘纤维软骨细胞具有较强的增殖能力,1～3代细胞可作为颞下颌关节盘组织工程的种子细胞.

Isolation and cultivation of goat temporomandibular joint disc cells

Objective:To study the isolation,culture and identification of the TMJ cells and to observe the biological characteristics of cultured fibrochondrocytes.Methods:The TMJ discs were dissected from two 1 month goats under sterile conditions and were digested with collagenase.The cells were collected.Morphological changes and attachment efficiency were constantly observed under phase-contrast microscope.Immunohistochemical staining for type I collagen as well as toluidine blue staining were performed.Ultrastruc...