慢性应激肝郁脾虚模型大鼠行为学变化及逍遥散调节作用

目的:研究分析慢性应激肝郁脾虚模型大鼠行为学变化及逍遥散的调节作用。方法:采用捆绑束缚方式建立大鼠模型,60只大鼠随机平均分为3组,正常对照组、模型组、模型+逍遥散组。观察各组大鼠一般状态、体质量、摄食量和旷场、高架十字实验行为学指标变化。结果:模型组大鼠在慢性束缚应激初期呈积极抵抗状态,继而抑郁,甚或呈绝望状态;旷场实验模型大鼠5 min内移动总路程、中央区移动距离均减少;高架十字试验5 min内模型组大鼠在开放臂内停留时间和进入次数较正常组减少,进入封闭臂次数减少而停留时间增加。说明慢性应激可引起动物探究性自主活动能力下降,随着应激时间的延长,动物的行为表现逐渐出现郁闷、淡漠、抑郁、焦虑状态,同时伴随着大鼠体质量和摄食量较正常组明显下降;逍遥散则对模型大鼠的上述改变具有一定的调节作用。结论:逍遥散可在一定程度上改善慢性应激肝郁脾虚模型大鼠的抑郁或焦虑状态,调节其体质量、摄食量及行为学的异常变化。

Ethological Changes of Chronic Stress Liver-qi and Spleen Deficiency Type Rats and Accomodation of Xiao Yao San

to study and analyze the ethologhical changes of chronic stress liver-qi and spleen deficiency type rats and the accomodation of Xiao Yao San ( XYS) . Methods:60 model rats, made by binding mode, were randomly divided into three groups:control group, model group and model&XYS group, observing the changes of their common states, mass, food consumption, the open field and elevated plus experimental behavior. Results:rats of model group showed a state of active resistance at the early stage of chro-nic binding stress, and further depression, or even desperation;rats in the open field test had shortened total moving distance and dis-tance in central zone in five minutes;in elevated plus experiment, in five minutes, the residence time in the open arm and entries of the rats reduced compared to control group;time in the closed arm lengthened while the entries reduced. It showed that chronic stress could decrease the animal's exploring ability of independent activities. With the lengthening of stress time, the rats became gloomy, in-different, depressed and anxious, accompanying with the decreasing of body mass and food consumption significantly;XYS could regu-late the changes mentioned above. Conclusion:XYS can in some degree improve the state of depression or anxiety in chronic stress liv-er-qi and spleen deficiency type rats, regulate the body mass, food consumption and abnormal behaviors.