Expression of caspase-9 affected by AG on retina of
rats with chronic IOP elevation |
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Authors: | Qing-Zhu Nie Qian Sh Ying-Shuang Wang Dong-Mei Gui Zhi-Li Liu and Dian-Wen Gao |
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Institution: | Department of Ophthalmology, Shengjing Hospital Affiliated to
China Medical University, Shenyang 110004, Liaoning Province,
China;Department of Ophthalmology, Shengjing Hospital Affiliated to
China Medical University, Shenyang 110005, Liaoning Province,
China;Department of Ophthalmology, Shengjing Hospital Affiliated to
China Medical University, Shenyang 110006, Liaoning Province,
China;Department of Ophthalmology, Shengjing Hospital Affiliated to
China Medical University, Shenyang 110007, Liaoning Province,
China;Department of Ophthalmology, Shengjing Hospital Affiliated to
China Medical University, Shenyang 110008, Liaoning Province,
China;Department of Ophthalmology, Shengjing Hospital Affiliated to
China Medical University, Shenyang 110009, Liaoning Province,
China |
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Abstract: | AIM: To study caspase-9 expression on rat retina in the
process of chronic elevation of IOP and the changes with the
application of amino guanidine (AG), thus to investigate the
potential protective function of AG to rat retina with chronic
elevation of IOP.
METHODS: Immunohistochemistry, RT-PCR and Western
blot were used to observe retinal morphology and the
expression of caspase-9 at different time points of rat with
chronic IOP elevation, both affected and not affected by the
application of AG.
·RESULTS: Compared with control group, as time passed
retina of experimental group gradually had detectable
morphological changes. On 21st day of chronic IOP elevation,
retinas became thinner and the quantity of retinal ganglion
cells (RGCs) decreased; caspase-9 expression increased,
consistent with the morphological changes. The group using
AG presented relatively smaller morphology changes and less
expression of caspase-9.
CONCLUSION: Apoptosis-related gene caspase-9 plays a
part in the process of chronic IOP elevation; AG protects
retina by down-regulating expression of caspase-9. |
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Keywords: | retina chronic IOP elevation apoptosis amino guanidine caspase-9 |
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