Expression of junctional adhesion molecule -1 in
human corneal epithelium |
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Authors: | Li-Zhong Chen Jing Hong Nobuyuki Ebihara and Akira Murakami |
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Abstract: | AIM: To investigate the expression and distribution of junction adhesion molecule-1(JAM-1) in human corneal epithelium and compare with those of occludin.
METHODS: The expression in RNAs of JAM-1 and occludin was revealed by RT-PCR and the presence of protein was analyzed by the FACS method. Double immunofluorescent staining was used to determine the tissue distribution of JAM-1 and occludin in human corneal epithelium.
RESULTS: The expression of JAM-1 and occludin was found in cultured human corneal epithelial cells. The double immunofluorescent study showed positive staining for JAM-1 at cell borders in the entire epithelial layer, while relatively extensive staining was seen in the superficial layer, where it coexisted with the expression of occludin.
CONCLUSION: JAM-1 is expressed in entire layer of human corneal epithelium encircling the cells. |
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Keywords: | junction adhesion molecule-1 occludin tight junction |
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