| 反义CD44基因转染对人眼小梁细胞合成细胞外基质的影响 |
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| 引用本文： | 李中国,王青,梅红英,罗一青,陈永娟,张宇宏,余萍,云亚歌,史强. 反义CD44基因转染对人眼小梁细胞合成细胞外基质的影响[J]. 国际眼科杂志, 2012, 12(4): 615-617 |
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| 作者姓名： | 李中国 王青 梅红英 罗一青 陈永娟 张宇宏 余萍 云亚歌 史强 |
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| 作者单位： | 中国江苏省南京市，南京医科大学附属南京医院（南京市第一医院）眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科;中国青海省西宁市，青海大学附属医院眼科 |
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| 基金项目： | 中国青海省自然科学基金资助项目（No.2009-Z-706） |
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| 摘 要： | 目的：观察反义CD44基因转染对人眼小梁细胞合成细胞外基质的影响，探讨黏附分子CD44在原发性开角型青光眼(primary open angle glaucoma, POAG)发病过程中可能的作用。 方法：采用硫代修饰的CD44反义寡核苷酸，通过脂质体介导转染体外培养的人眼小梁细胞，免疫组织化学染色观察CD44反义寡核苷酸对小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白的影响，放射免疫法观察CD44反义寡核苷酸对小梁细胞合成透明质酸的影响。结果：CD44反义寡核苷酸对人眼小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白起促进作用，且呈浓度依赖性，浓度越高促分泌作用越强,但对小梁细胞合成透明质酸起抑制作用，浓度越高抑制作用越明显。结论：CD44反义寡核苷酸封闭CD44基因表达后人眼小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白增加而合成透明质酸减少。黏附分子CD44可能通过影响小梁细胞合成细胞外基质功能参与了POAG发病过程。
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| 关 键 词： | 小梁细胞；CD44；反义寡核苷酸；细胞外基质蛋白 |
| 收稿时间： | 2012-01-12 |
| 修稿时间： | 2012-02-29 |
Effect of CD44-specific antisense oligonucle- otide on extracellular matrix synthesis of human trabecular meshwork cells |
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| Zhong-Guo Li,Qing Wang,Hong-Ying Mei,Yi-Qing Luo,Yong-Juan Chen,Yu-Hong Zhang,Ping Yu,Ya-Ge Yun and Qiang Shi. Effect of CD44-specific antisense oligonucle- otide on extracellular matrix synthesis of human trabecular meshwork cells[J]. International Eye Science, 2012, 12(4): 615-617 |
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| Authors: | Zhong-Guo Li Qing Wang Hong-Ying Mei Yi-Qing Luo Yong-Juan Chen Yu-Hong Zhang Ping Yu Ya-Ge Yun Qiang Shi |
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| Affiliation: | Department of Ophthalmology, Affiliated Nanjing First Hospital of Nanjing Medical University,Nanjing 210006, Jiangsu Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China;Department of Ophthalmology, Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China |
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| Abstract: | AIM:To observe the effect of CD44-specific antisense oligonucleotide on extracellular matrix(ECM) synthesis of human trabecular meshwork cells,then to discuss the possible relationship between CD44 and primary open angle glaucoma(POAG).METHODS:CD44-specific antisense oligonucleotide were delivered with cationic lipid to cultured human trabecular meshwork cells.Effect of CD44-specific antisense oligonucleotide on synthesis of collagen-Ⅰ,Laminin were examined by immunohistochemistry and the synthesis of hyaluronic acid were examined by radioimmunoassay.RESULTS:Three different concentrations of CD44 antisense oligonucleotide could promote the synthesis of collagen-Ⅰ,Laminin in trabecular meshwork cells and in a concentration-dependent manner.But CD44 antisense oligonucleotide inhibited the synthesis of hyaluronic acid.CONCLUSION:CD44 antisense oligonucleotide promotes the synthesis of collagen-Ⅰand Laminin but inhibits the synthesis of hyaluronic acid.CD44 may play a role in pathogenesis of POAG by affecting the synthesis of ECM. |
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| Keywords: | trabecular meshwork cell CD44 antisense oligonucleotide extracellular matrix |
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