Effect of Fat Feeding on Pro-oxidant and Anti-oxidant Enzyme Systems in Rat Intestine: Possible Role in the Turnover of Enterocytes |
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Authors: | Aasma Turan Ravinder Gill Pradeep K Dudeja Harsh Mohan Akhtar Mahmood |
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Institution: | (1) Department of Biochemistry, Panjab University, Chandigarh, 160014, India;(2) Department of Medicine, University of Illinois, Chicago, IL 60612, USA;(3) Department of Pathology, Government Medical College, Chandigarh, 160032, India |
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Abstract: | Immature epithelial cells generated in the crypt base undergo differentiation while progressing to the villus tip, where the
cells upon apoptosis are detached from the underlying muscular tissue. We previously reported that lipid peroxidation might
be involved in the turnover of enterocytes across the crypt–villus axis in rat intestine (Dig Dis Sci 52:1840–1844, 2007).
To examine whether long-term feeding of fat with different fatty-acid composition influences this process, in the present
study we investigated the effect of feeding fish oil (n − 3) and corn oil (n − 6) polyunsaturated fatty acids on lipid per-oxidation and anti-oxidant systems in different epithelial cell fractions isolated
in rat intestine. Feeding fish oil or corn oil markedly enhanced lipid per-oxidation levels of enterocytes throughout villus
height compared with control, but there was no difference in the distribution profile of pro- and anti-oxidant enzyme systems
and lipid per-oxidation across the crypt–villus axis under these conditions. Analysis of lipid peroxidation levels in different
cell fractions revealed that the thiobarbituric acid reactive substance were 9- to 11-fold higher at the villus tip compared
with at the crypt base. The activities of glutathione reductase and glutathione-S-transferase were 2- to 5-fold higher in
villus tip compared to the crypt region. However, the activities of superoxide dismutase and catalase were 6- to 8-fold high
at the crypt base compared with at villus tip cells. Immunocytolocalization of superoxide dismutase showed high staining in
crypt base compared with that in villus, tip cells. These findings further suggest that generation of reactive oxygen species
in enterocytes across the crypt–villus axis may be involved in turnover of enterocytes across the crypt–villus unit in rat
intestine. |
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Keywords: | Fat feeding Pro-oxidant enzymes Lipid peroxidation Rat intestine |
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