网状分枝扩增法检测非霍奇金淋巴瘤外周血中EB病毒基因的研究

目的探讨网状分枝扩增法（RAM）检测非霍奇金淋巴瘤（NHL）外周血EB病毒基因在EB病毒感染与NHL发病的相关性研究中的意义.方法用人工合成EBER-1启动子基因,建立网状分枝扩增检测法,以EB病毒阳性细胞株Raji和EB病毒阴性的人白血病细胞株NB4作对照,检测120例NHL患者外周血中EB病毒基因.结果 RAM最少能够检出样品中10拷贝/μl的EB病毒DNA,与多重聚合酶链反应（mPCR）的灵敏度一致;并从120例NHL患者中,检出76例阳性（63.3%）,与mPCR方法的阳性检出符合率为100%.结论 RAM法具有高度敏感度和特异性、操作简便,且可在等温条件下扩增核酸;同时证明NHL发病与EB病毒感染具有较高的相关性.

Study of detection of EB virus in peripheral blood of patients with non-hodgkin lymphoma by ramification amplification method

Objective To detect EB virus in peripheral blood of patients with non-hodgkin lymphoma (NHL) by ramification amplification method (RAM).To investigate the relativity of NHL with infection of EB virus.Methods RAM was used to detect EB virus with the man-made target gene sequence of promotor of EBER-1. In 120 cases of patients with NHL, EB virus were detected by RAM, and the result of RAM was compared with that of PCR.Raji cells and NB4 cells were used as positive control and negative control respectively.Results RAM could detect 10 molecular target gene of the exponent.76 cases were positive in 120 cases of NHL patients and the positive rate was 63.3%, The result is identical with that of PCR.Conclusion Ramification amplification method is sensitive, convenient and easy to perform.NHL has a high relativity with infection caused by EB virus.