大鼠肝移植术后输注ω-3多不饱和脂肪酸对T淋巴细胞的影响

目的 探讨大鼠肝移植术后应用ω-3多不饱和脂肪酸对移植肝脏功能、受体T淋巴细胞和急性排斥反应的影响.方法 行BN(RTln)大鼠到Lewis(RT11)二袖套法大鼠肝移植18例,术后颈内静脉插管,微量泵匀速输注液体.生理盐水NS组(n=6)输注生理盐水,肠外营养PN组(n=6)输注营养液,脂肪酸OM组(n=6)输注营养液+ω-3脂肪乳.术后第7天处死大鼠,留取肝组织标本病理检查,取血清检测生化指标.取抗凝血0.5 ml,流式细胞仪检测CD4+、CD8+、CD4+CD25+T细胞和CD8+CD28-T细胞.结果 大鼠肝移植术后输注ω-3脂肪酸的OM组CD4+、CD8+、CD4+CD25+和CD8+CD28-T细胞下降,与Ns组比较差异有统计学意义(t=4.28,P<0.01;t=2.63,P<0.05;t=2.59,P<0.05;t=8.86,P<0.01).OM组CD4+、CD8+、CD4+CD25+和CD8+CD28-T细胞下降,与PN组比较差异有统计学意义(分别t=8.06,t=3.57,t=5.35.t=7.98,均P<0.01).OM组CD4+/CD8+比值下降,与NS组和PN组比较差异有统计学意义(分别t=2.41,t=8.74,均P<0.01).病理学检查移植肝排斥反应强度(rejection activity index,RAI)评分,NS组为7.17±0.98,PN组为6.17±0.75,OM组为4.33±0.52,OM组与NS组、PN组比较排斥反应减轻(分别t=6.25,t=4.92,均P<0.01).结论 静脉输注ω-3多不饱和脂肪酸可以抑制T淋巴细胞,显著降低CD4+辅助性T细胞比例,减轻排斥反应.抑制性T细胞和调节性T细胞与ω-3脂肪酸减轻大鼠肝移植排斥反应的效应无关.

Effects of omega-3 polyunsaturaed fatty acid transfusion on T lymphocytes in liver transplantation rats

Objective To evaluate the effect of omega-3 polyunsaturaed fatty acid on liver function, donor's T lymphocytes and acute rejection following orthotopic liver transplantation in rats. Methods Orthotopie liver transplantation from BN (RT1 n) to Lewis was performed. Normal saline (NS, n = 6), parenteral nutrition (PN, n = 6) or parenteral nutrition and Omega-3 fatty acid (OM, n = 6) was given through internal jugular vein catheter respectively beginning the first day for consecutive 6 days. At 7thday, the liver function and pathological changes of the transplant liver were observed. The percentage of CD4+ , CD8+, CD4+ CD25+ and CD8+ CD28- T lymphocytes were analyzed by flow-cytometry. Results In OM group the CD4+, CD8+ , CD4+ CD25+ and CD8+ CD28- T lymphocytes significantly decreased, compared with NS (t = 4.28, P < 0.01 ; t = 2.63, P < 0.05 ; t = 2.59, P < 0.05 ; t = 8.86, P <0.01)or PN group(t =8.06, P<0.01; t =3.57, P<0.01; t =5.35, P<0.01; t =7.98, P<0.01). The ratio of CD4+ to CD8+ significantly decreased in OM group compared with NS group and PN group(t = 6.25, P<0.01; t=4.92, P<0.01). RAI (rejection activity index) in NS group, PN group and OM group was 7.17 +0.98, 6.17±0.75 and 4.33±0.52, respectively. The rejection grade in OM group was siguificanfly alleviated than that in NS group and PN group(t =6.25, P <0.01;t =4.92, P <0.01). Conclusion Omega-3 fatty acid inhibits T lymphocytes, especially help T lymphocytes, alleviates acute rejection. The suppressor T cell and the regulatory T cell were not responsible for the alleviation of the rejection.