灵芝孢子粉对癫痫大鼠脑细织中SS、5-HT和ATP酶的影响

目的观察灵芝孢子粉对戊四氮致痫大鼠脑组织中生长抑素（ss）、5一羟色胺（5-HT）和ATP酶的影响，探讨灵芝孢子粉的抗癫痫作用机制。方法健康雄性Wistar大鼠30只，分为空白对照组、癫痫模型组、灵芝孢子粉组，每组10只。癫痫模型组采用戊四氮腹腔注射＋0．9％NaCl溶液灌胃；灵芝孢子粉组采用戊四氮腹腔注射＋灵芝孢子粉灌胃；空白对照组采用0．9％NaCl溶液腹腔注射＋0．9％NaCl溶液灌胃。各组分别干预28天后断头取脑，行免疫组化染色法和比色法，观察脑组织中SS、5-HT和ATP酶含量变化。结果①灵芝孢子粉组和癫痫模型组均达到癫痫模型“点燃”标准。②免疫组织化学染色显示：癫痫模型组大脑皮质及海马SS免疫反应阳性细胞数比空白对照组明显增多，差异具有统计学意义（P〈0．01）；灵芝孢子粉组大脑皮质及海马SS免疫反应阳性细胞比癫痫模型组数减少，差异具有统计学意义（P〈0．01）；③癫痫模型组海马5-HT含量明显低于空白对照组，差异具有统计学意义（P〈0．01）；灵芝孢子粉组海马5-HT含量比癫痫模型组增多，差异具有统计学意义（P〈0．05）。④癫痫模型组大脑皮质及海马ATP酶含量低于空白对照组，差异具有统计学意义（P〈0．01）；灵芝孢子粉组高于癫痫模型组，差异具有统计学意义（P〈0．05）。结论灵芝孢子粉能有效降低癫痫大鼠脑皮质和海马区SS的含量，同时能提高脑组织中5-HT和ATP酶的含量，使神经元兴奋性减弱，减轻癫痫的发作，从而降低癫痫发作给神经系统带来的损伤。

Effect of ganoderma lucidum spores on the somatostatin and 5-HT in brain of epilepsy rats

Objective To observe the effect of ganoderma lucidum spores on the SS, 5-HT and ATPase, and to explore its anti-epileptic mechanism. Methods Thirty healthy Wistar rats were divided into blank control group, epilepsy model group, ganoderma lucidum spores group, ten in each group. The rats in epilepsy model group were treated by pentylenetetrazol intraperitoneal injection ＋ intragastric administration of NS; ganoderma lucidum spores group were treated by pentylenetetrazol intraperitoneal injection ＋ ganoderma lucidum spores; the blank control group were treated by NS intraperitoneal injection ＋ intragastric administration of NS. The brain was cut down and took the brain after 28 days. The contents of SS, 5-HT and ATPase were detected by immunohistochemical staining and chemical chromatometry method. Results ① The ganoderma lucidum spores group and epileptic model group reached the kindled standard of epilepsy models. ② Immunohistostaining shows ： compared with the blank control group, the number of somatostatin immunological reacted positive cells in cerebral cortex and hippocampus in the epilepsy model group were significantly increased （P 〈 0. 01 ） ; compared with the epilepsy model group, the number of somatostatin immunological reacted positive cells in cerebral cortex and hippocampus in ganoderma lucidum spores group were significantly decreased （P 〈 0.01 ） ; ③ The content of 5-HT in hippocampns were significantly decreased in epilepsy model group, and which was different from blank control group （ P 〈 0.01 ） ; the content of 5-HT in hippocampus were significantly increased in ganoderma lucidum spores group, which was different from epilepsy model group （ P 〈 0.05 ）. ④ The content of ATPase in cerebral cortex and hippocampus were significantly decreased in the epileptic model group, which was difference from blank control group （P 〈 0.01 ） ; the content of ATPase in cerebral cortex and hippocampus were significantly increased in the ganoderma lucidum spores group, which was different from epilepsy model group （ P 〈 0.05 ）. Conclusion The gmloderma lucidum spores can reduce the SS content in cerebral cortex and the hippocampus and increase the content of 5-HT and ATPase. It can decrease the neuron excitability, alleviate epilepsy and relieve the injury of nervous system induced by epilepsy.