加味盆痛灵对子宫内膜异位症模型大鼠血清及腹腔液IL-1β、NGF的影响

目的探讨加味盆痛灵方抑制子宫内膜异位症（EMs）疼痛的机制。方法SD大鼠自体内膜移植建立EMs模型。将造模成功的大鼠随机分为模型组、盆痛灵组、加味盆痛灵组、孕三烯酮组、消炎痛组，每组12只，另设正常组12只。各组给予相应的干预措施。采用ELISA法检测大鼠血清及腹腔液中白细胞介素-1β（IL-1β）、神经生长因子（NGF）的含量。结果与正常组比较，模型组血清及腹腔液中的IL-1β、NGF水平升高，差异有统计学意义（P〈0．05）。与模型组比较，各治疗组血清及腹腔液中NGF水平均降低（P〈0．05），盆痛灵组、加味盆痛灵组血清中IL-1β水平下降，孕三烯酮组和消炎痛组治疗后血清及腹腔液中IL-1β水平下降（P〈0．05）。结论血清及腹腔液中IL-1β、NGF水平的改变与子宫内膜异位症疼痛的发生有关，加味盆痛灵可能通过降低IL-1β、NGF的水平，达到治疗子宫内膜异位症疼痛的目的。

Effect of ＂Jiawei Pentongling＂ on IL-1 β and NGF in serum and peritoneal fluid in EMs rats

Objective To study the mechanism of ＂Jiawei Pentongling＂ against pain in EMs. Methods The models were built and divided into five groups： model group, ＂Pentongling＂ group, ＂Jiawei Pentongling＂ group, gestrinone group and ＂Xiaoyantong＂ group, 12 rats in each group. The normal group was built with 12 rats. The rats in each group were treated by corresponding interventions. The contents of IL-1β and NGF were detected by ELISA. Results Compared with normal group, the levels of IL-1β and NGF in serum and peritoneal fluid in model group were increased, with significant difference between the two groups （P 〈 0.05 ）. Compared with model group, the NGF level in serum and peritoneal fluid in each treatment group was decreased, with significant difference between model group and other treatment groups （P 〈 0.05 ）. The IL-1β level in serum of ＂Pentongling＂ group and ＂Jiawei Pentongling＂ group were decreased, and in serum and peritoneal fluid of gestrinone group and ＂Xiaoyantong＂ group were decreased, with significant difference between them （ P 〈 0.05 ）. Conclusion The changes of IL-1β and NGF in serum and peritoneal fluid relate to the onset of pain in EMs. ＂Jiawei Pentongling＂ can lower the IL-1β and NGF level to treat EMs.