双重血流同时开放减轻大鼠肝移植胆管I/R损伤的研究

目的 探讨门静脉、肝动脉双重血流同时开放对大鼠肝移植胆管缺血/再灌注(I/R)损伤的保护作用.方法 选用雄性SD大鼠建立大鼠自体原位肝移植模型,根据肝脏恢复血供时门静脉、肝动脉血流是否同时开放,将大鼠随机分为双重血流同时开放组(P组)和门静脉先开放组(N组),另设假手术作对照组(S组).分别于供肝再灌注后2、6及24h处死各组动物.检测血清ALT、AST、GGT、AKP、TBiL及DBiL水平,HE切片作病理组织学检查,比色法测定髓过氧化物酶(MPO)的含量,RT-PCK法检测胆管组织TNF-a及ICAM-1 mRHA的表达.结果 肝脏再灌注后6、24 h两个时点P组的GGT水平明显低于N组水平(P＜0.05);肝脏再灌注后24 h.P组的AKP、TBiL、DBiL水平及胆管损伤病理学评分明显低于N组水平(P＜0.05);再灌注后6 h,N组大鼠肝组织的MPO含量明显高于P组(P＜0.05);供肝再灌注后2 h、6 h,P组大鼠肝组织TNF-a及ICAM-1 mRNA的相对表达水平明显低于N组(P＜0.05).结论 门静脉、肝动脉双重血流同时开放.有利于减轻肝移植物胆管组织的I/R损伤;其机制可能与TNF-a和ICAM-1表达水平的降低以及中性粒细胞浸润的减少有关.

Effects of simultaneuos revascularization on bile duct ischemia/reperfusion injury in rats liver transplantation

Objective To investigate the effects of simultaneuos hepatic artery and portal revascularization on bile duct ischemia/reperfusion injury in rats liver transplantation.MethodsMale Sprague-Dawley rats were used to establish an autologous orthotopic liver transplantation model.According to whether performing simultaneuos hepatic artery and portal revascularization, simultaneuos revascularization group(group P) and portal vein revascularization group(group N) were divided randomly.The animals were separately k...