雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响

目的：探讨雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响。方法：雌性SD大鼠卵巢切除术（OVX）后1周,随机分成3组：OVX＋雌激素（0.15mg/kg,s.c.）、OVX＋生理盐水、对照组。4周后,Westernblotting检测α和β两种雌激素受体在心肌组织中的表达。结果：卵巢切除后,血清雌激素水平与对照组[（88±22vs403±59）pmol/L,P〈0.05]相比明显下降,左心室肌中两种雌激素受体表达均下降（P〈0.05）。给予雌激素后,血清雌激素水平上升为（3864±105）pmol/L,雌激素β受体表达增多（P〈0.05）,α受体表达下降（P〈0.05）。结论：雌激素改变卵巢切除后雌性大鼠心肌组织中雌激素受体的表达。

Effects of estrogen on estrogen receptors in myocardium of ovariectomized female rats

AIM： To investigate the expression of estrogen receptors in myocardium of ovariectomized female rats. METHODS： One week after bilateral ovariectomy, the female SD rats were divided into 3 groups randomly： OVX ＋ estrogen （ 0.15 mg/kg, s.c. ）, OVX ＋ normal sodium and control. After 4 weeks of treatment, the expression of estrogen receptors in the myocardium were evaluated by Western blotting. RESULTS： After ovariectomy, sennn estrogen decreased significantly, compared with control [ （ 88 ± 22 vs 403 ± 59） pmol/L, P 〈 0.05 ], the expression of estrogen receptors decreased （ P 〈 0.05）. After estrogen replacement therapy, serum estrogen increased [ （3864 ± 105） pmol/L], estrogen upregulated the expression of ERt3 receptor（ P 〈 0.05） and downregulated ERa receptor （P 〈 0.05） in myocardium. CONCLUSION： Estrogen replacement modifies the expression of estrogen receptors in myocardium of ovariectomized female rats.