阿霉素诱导人胆囊癌细胞凋亡的研究

目的　观察阿霉素 (ADM )对胆囊癌细胞凋亡的影响。方法　在体外培养的胆囊癌细胞中加入不同浓度的阿霉素 ,应用光镜、电镜、DNA凝胶电泳及流式细胞仪检测胆囊癌细胞凋亡的形态学特征、生化学特征、细胞凋亡百分率及细胞周期的变化。结果　在阿霉素作用下 ,凋亡的胆囊癌细胞出现膜小泡、凋亡小体等特征性改变 ;DNA电泳呈现典型的“梯状”条带 ;流式细胞仪测定 ,出现典型的凋亡峰 ,其凋亡百分率随着药物浓度的提高而明显升高 ,分别与对照组比较 ,差异有显著性 (P <0 .0 5 ) ;同时 ,S期细胞含量下降 ,而G2 /M期细胞含量上升。非凋亡细胞则无此表现。结论　ADM可诱导胆囊癌细胞发生凋亡 ,并可使胆囊癌细胞生长受阻于G2 /M期 ,这可能是其杀伤肿瘤的一种重要机制

Apoptosis of human gallbladder carcinoma GBC-SD cells induced by adriamycin in

Objective To study the effect of adriamycin on apoptosis in human gallbladder carcinoma.Methods Human cultured gallbladder carcinoma cell line GBC SD was exposed in vitro to different concentrations of adriamycin for various periods.Cell apoptosis was ascertained by cell morphology under light and electron microscopy,and DNA electrophoresis and flow cytometry.Results When GBC SD cells were treated with adriamycin at different concentrations,cell apoptosis was induced.Typical apoptotic bodies appeared and DNA ladder could be demonstrated on DNA electrophoresis.Apoptotic peak was also shown by flow cytometry.The percentage of cell apoptosis was increased by adriamycin in a concentration dependent manner as compared with the control group ( P <0.05).The cells in S phase of cell cycle were decreased and those in G 2/M phase increased.Conclusion Adriamycin successfully induced the apoptosis of human gallbladder carcinoma cells,and arrested them in G 2/M phase,which may be an important mechanism for killing turmor.