维生素E对高脂血症小鼠Thl和Th2细胞因子表达的影响

目的观察维生素E对高脂血症小鼠辅助性T淋巴细胞l（Thl）和辅助性T淋巴细胞2（Th2）细胞因子表达的影响。方法将50只ICR雄性性成熟小鼠随机分为A、B、C、D、E5组，每组10只，分别给予高脂饲料、不同浓度维生素E（每日10mg／kg、20mg／kg、40mg／kg）加高脂饲料及普通饲料连续喂养4周，后处死小鼠抽取静脉血观察比较血清Thl和Th2细胞因子表达情况肿瘤坏死因子-α（TNF-α）、白细胞介素-12（IL-12）及白细胞介素4（IL4）、白细胞介素．5（IL-5）]及甘油三酯（TG）、胆固醇（TC）浓度。结果A组血清TNF-α、IL-12及TG、TC浓度均高于B、C、D、E组，血清IL-4及IL-5浓度均低于B、C、D、E组，差异均有统计学意义（P〈0．05）。B、C、D3组血清TNF-α、IL-12及TG、TC浓度均随维生素E剂量增加逐渐降低，血清IL-4及IL-5浓度随维生素E剂量增加逐渐升高，且3组间比较差异均有统计学意义（P〈0．05）。E组血清TNF-α、IL-12及TG浓度均低于B、C、D组，血清TC浓度低于B、C组，血清IL-4及IL-5浓度均高于B、C、D组，除D、E2组血清IL-4比较差异无统计学意义外（P〉0．05），余差异均有统计学意义（P〈0．05）。血清IL-5浓度与IL-12浓度呈负相关关系。结论维生素E能抑制高脂血症所导致的免疫损伤与炎性反应，并可降低血脂。

Effect of Vitamin E on Expressions of Thl and Th2 Cytokines in Mice with Hyperlipidemia

Objective To explore the effect of Vitamin E on expressions of helper T cells （Thl and Th2） in mice with hyperlipidemia. Methods A total of 50 viripotent male ICR mice were randomly divided into group A, B, C, D and E （n = 10）. Mice fed on high-fat food in group A, B, C and D, and group B, C and D were lavaged with Vitamin E in different concentrations （10 mg/kg, 20 mg/kg and 40 mg/kg at each day） respectively for 4 weeks, while control group was fed on normal diet for 4 weeks, and then all the mice were sacrificed. The expressions of Thl and Th2 cytokines tumor necrosis fac- tor a （TNF-α） , interleukin 12 （IL-12） , interleukin 4 （IL-4） and interleukin 5 （IL-5）] and the concentrations of triglyeer- ide （TG） and cholesterol （TC） in serums of venous blood in the five groups were detected and compared. ReSults The con- centrations of TNF-α, IL-12, TG and TC in group A were significantly higher than those of group B, C, D and E （P 〈 0.05 ） , while the eoncentrations of IL-4 and IL-5 in group A were significantly lower than those of group B, C, D and E （ P 〈 0.05 ）. In group B, C and D, the concentrations of TNF-α, IL-12, TG and TC were decreased gradually with increasing con- centration of Vitamin E, while the concentrations of IL-4 and IL-5 were increased gradually with increasing concentration of Vitamin E, and the differenees among the three groups were statistically significant （P 〈 0.05 ）. In group E, concentrations of TNF-et, IL-12, and TG were lower than those of group B, C and D, TC concentration was lower than those of group B and C, concentrations of IL-4 and IL-5 were higher than those in group B, C and D, and the differences were statistically significant （ P 〈 0.05 ） except for the difference of serum IL--4 concentration between group D and E （ P 〉 0.05 ）. IL-5 concentration was negatively related with the IL-12 concentration. Conclusion Vitamin E can inhibit the immune injury and inflammatory reac- tion induced by hyperlipemia and bring down lipid level.