不同地区蜂胶原料药的质量评价 |
| |
引用本文: | 贾艾玲,郑爱竹,王睿,等.不同地区蜂胶原料药的质量评价[J].辽宁中医杂志,2014(2):316-318. |
| |
作者姓名: | 贾艾玲 郑爱竹 王睿 等 |
| |
作者单位: | 长春中医药大学,吉林长春130117 |
| |
基金项目: | 国家自然科学基金项目(81274092) |
| |
摘 要: | 目的:建立不同产地蜂胶的质量评价方法。方法:采用薄层色谱法,以白杨素和高良姜素为对照品,甲苯-乙酸乙酯-甲酸(10∶4∶3)为展开剂,进行定性鉴别研究;采用高效液相色谱法,色谱柱Dikma Technologies(250 mm×4.6mm,5μm);流动相甲醇-0.15%磷酸水溶液(62∶38);流速1.0 mL·min-1;柱温25℃;检测波长268 nm。结果:28个产地蜂胶原料药中白杨素和高良姜素的含量均符合《中国药典》(2010年版)的规定的仅有6个地区,大部分地区蜂胶原料药中高良姜素含量符合标准,而白杨素含量不达标。结论:不同产地蜂胶原料药中白杨素和高良姜素的含量不同,建立的定性、定量方法准确可靠,专属性强,适合不同产地蜂胶原料药的质量控制。
|
关 键 词: | 蜂胶 白杨素 高良姜素 薄层色谱法 高效液相色谱法 |
Quality Evaluation of Propolis Contents in Different Regions |
| |
Institution: | JIA Ailing, ZHENG Aizhu, WANG Rui, Q1u Zhidong (Changchun University of Chinese Medicine, Changchun 130117 ,Jilin, China) |
| |
Abstract: | Objective:To establish the methods of Propolis content determination from different regions. Methods:Thin layer chromatography (TLC)was adopted, using chrysin and galangin as reference substance and toluene, ethyl acetate -formic acid( 10 :4:3 )as developing agent, to study propolis of different regions. High performance liquid chromatography (HPLC)method was a- dopted,using octadeeyl silane bonded silica as stationary phase. Methanol 0.15% phosphoric acid aqueous solution( 62:38 )was as mobile phase ; setting flow rate was 1.0 ml/min, detection wavelength was 268 nm, to determine the contents of chrysin and ga- langin in propolis from different regions. Results : There were only six areas where the galangin and chrysin met the standards given by Pharmacopoeia of China(2010 version)from 28 samples of different regions. Conclusion:Contents of ehrysin and galangin from propolis of different origins vary. The established quantitative and qualitative methods are accurate, reliable and exclusive, provi- ding a basis for the quality control of propolis and its pharmaceutical preparation. |
| |
Keywords: | propolis chrysin galangin thin layer chromatography high performance liquid chromatography |
本文献已被 CNKI 维普 等数据库收录! |
|