| 冠通方对急性心肌梗死大鼠心肌梗死面积及心肌组织CRP mRNA表达的影响 |
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| 引用本文： | 何劲松,曲楠,刘婧,等. 冠通方对急性心肌梗死大鼠心肌梗死面积及心肌组织CRP mRNA表达的影响[J]. 辽宁中医杂志, 2014, 0(1): 167-169 |
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| 作者姓名： | 何劲松 曲楠 刘婧 等 |
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| 作者单位： | [1]广西中医药大学附属瑞康医院心内科,广西南宁530011 [2]广西中医药大学研究生,广西南宁530011 [3]广西中医药大学方剂教研室,广西南宁530011 [4]广西中医药大学科研处,广西南宁530011 |
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| 基金项目： | 广西自然科学基金（2010GXNSFA013222） |
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| 摘 要： | 目的:探讨冠通方对急性心肌梗死(AMI)大鼠心肌梗死面积及心肌组织中CRP mRNA表达的影响。方法:20只成功造模的急性心肌梗死SD大鼠随机分为模型组、冠通方组,每组10只。空白组10只SD大鼠不作处理,假手术组10只开胸但不结扎冠脉。冠通方组冠通方灌胃,各组均正常饲料及饮水喂养,8周后处死大鼠。采用2,3,5-氯化三苯基四氮唑(TTC)法测量心肌梗死面积,逆转录聚合酶链反应(RT-qPCR)方法测定各组心肌组织中C反应蛋白(CRP)的mRNA表达结果。结果:(1)假手术组出现心肌损伤并未出现明显心肌梗死,冠通方组同模型组比较心肌梗死面积稍有下降,但无统计学意义(P>0.05)。(2)与空白对照组比较,模型组CRP mRNA表达量显著升高(P<0.01)。(3)与空白对照组比较,假手术组CRP mRNA表达量升高,无显著性差异(P>0.05)。(4)冠通方组与模型组相比,CRP mRNA表达量显著降低(P<0.05)。结论:CRP参与了心肌梗死后的炎症反应,中药冠通方对大鼠心肌梗死面积无明显影响,对心梗后CRP mRNA表达有一定抑制作用。
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| 关 键 词： | 心肌梗死面积 冠通方 C反应蛋白 RT-qPCR 逆转录聚合酶链反应 TTC |
Effect of Guantong Formula on Cardiac Tissue Expression of CRP mRNA and Infarction Area in Acute Myocardial Infarction Model Rats |
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| Affiliation: | HE Jingsong1, QI Nan1,LIU Jing2, BEI Faweng2 ,YANG Xiaoying1,YI Zigang3, YUE Gueihua4 (1. Cardiology Department of Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530011, Guangxi, China; 2. Master Degree Candidate of Guangxi University of Chinese Medicine, Nanning 530011, Guangxi, China; 3. Formula ology Department of Guangxi University of Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530011, Guangxi, China; 4. Scientific Research Department of Guangxi University of Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530011, Guangxi, China) |
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| Abstract: | Objective ： To explore the effect of Guantong Formula on cardiac tissue expression of CRP mRNA and infarction are- a in acute myocardial infarction （AMI） model rats. Methods ：20 rats successful suffering AMI model were randomized into Guan- tong Formula group and AMI model group （n = 10 each）. 10 rats accepting false operating composed false operating group and another 10 rats were the blank control group. The Guantong Formula group was treated with Guantong Formula. The other was fed normally. The expression of CRP mRNA in rats＇ cardiac tissue was dedeminated with RT -qPCB two months later. And the infarc- tion area was detected with TTC two months later. Results ： （ 1 ） The cardiac tissue suffered slightly injury had no infarction in the false operating group. The infarction area slightly decreased in the Guantong Formula group than that in AMI group. They had no difference（P 〉 0.05 ）. （2）The comparative expression of CRP mRNA was rising obviously in AMI model group more that in blank control group（ P 〈 0. 01 ）. （3）The comparative expression of CRP mRNA was rising in the false operating group comparing with that in the blank group. But they was no difference（P 〉 0. 05 ）. （4） The comparative expression of CRP mRNA in Guantong Formula group was significantly lower than that in AMI model group（P 〈 0. 01 ）. Conclusion：The CRP participates in the inflam- mation after rats AMI and the Guantong Formula is effective in preventing the expression of CRP mRNA. Guantong Formula can decline AMI area in AMI model rat. |
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| Keywords: | myocardial infarction area Guantong Formula CRP mRNA RT - qPCR TTC |
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