| HPLC法测定鼻炎丸中黄芩苷的含量 |
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| 引用本文： | 张书燕,张辉.HPLC法测定鼻炎丸中黄芩苷的含量[J].齐鲁药事,2009,28(11):656-657. |
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| 作者姓名： | 张书燕 张辉 |
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| 作者单位： | 1. 山东省中医药大学附属医院,山东,济南,250014
2. 山东省立医院,山东,济南,250022 |
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| 摘 要： | 目的建立高效液相色谱法测定鼻炎丸中黄芩苷的含量.方法高效液相色谱法条件:色谱柱以十八烷基硅烷键合硅胶为填充剂;以甲醇-水-磷酸(47∶53∶0.2)为流动相;检测波长为280nm.结果黄芩苷在进样量为0.204～1.02μg(r=0.9999)范围内与峰面积呈良好的线性关系;测定本品中黄芩苷的含量,加样回收率为99.97%(n=6),RSD为0.70%.结论所建立的方法准确可行,重复性好,可有效控制鼻炎丸的质量.
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| 关 键 词： | 高效液相色谱法 鼻炎丸 黄芩苷 含量测定 |
Determination of baicalin in Biyan Pill by HPLC |
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| Abstract: | OBJECTIVE To develop an HPLC method for determining the content of baicalin in Biyan Pill.METHODS The high performance liquid chromatography was used to analyze quantitatively the content of baicalin with a column of C18,the mobile phase was methanol-water-phosphoric acid (47∶53∶0.2),the detection wave length was 280nm.RESULTS The results showed a good linearity in the range of 0.204～1.02μg (r=0.9999).The average recovery was 99.97%(n=6)and RSD was 0.70%.CONCLUSION This method was precise and reliable to determine baicalin in Biyan Pill. |
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| Keywords: | HPLC Biyan Pill baicalin determination |
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