2种兔颈总动脉缝合方法的比较研究

目的从操作时间、通畅情况及组织形态学方面探讨连续性血管吻合与间断性血管吻合之间建立动物动脉吻合模型的优劣。方法新西兰大白兔20只随机分为2组,以单侧颈总动脉为实验对象,通过连续性血管吻合和间断性血管吻合方法吻合兔颈总动脉,观察吻合后28 d血管组织病理形态学变化HE染色观测内膜厚度和中膜厚度并行计算机图像分析。对比其在吻合时间、通畅程度、组织形态学的变化。结果与间断组相比,连续组更为节省手术时间,对血管损伤相对较小,血管通畅率较间断吻合略低,2组内膜增生程度无显著性差异。结论连续缝合方式对术者的操作熟练度要求较高,虽然手术操作用时较间断吻合短,但较易形成吻合口狭窄影响造模效果,综合利弊2种造模方式效果基本相同,各有利弊。

Comparative study on two different suture techniques for rabbit arteria carotis

Objective It is to approach the fit and unfit of continuity and discontinuity vascular anastomosis to build animal model by examining the operation time,patency rate and pathology features.Methods Forty New Zealand rabbit were randomly divided into 2 groups.Twenty bisected arteries(one from each animal) in Group I and II were sutured with the interrupted suture and continuous suture respectively.The materials were harvested on 2 weeks after operation.The artery was investigated by histo-morphometrical and immunohistochemical approach: The area of intima and medial were calculated from sections stained with HE by computer image analysis.The operation time,patency rate and pathology features changes were assessed.Results Compared with interrupted suture,continuous suture was more time-saving and less vascular injury,and the rate of patency is little lower.There was no significant difference in the degree of intimal hyperplasia.Conclusion The both continuous suture techniques has advantages of less time and little damage,but it requires more skill.More easily formed anastomotic stricture.There is no difference between two type's suture techniques for rabbit arteria carotis.