层粘连蛋白反义RNA对TGFβ1调节五种细胞内信号分子？…

目的 明确转化生长因子（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒ，β１ＴＧＦβ１）对大肠癌细胞Ｅ－钙粘着蛋白，α－，β－，γ－连环蛋白及粘着斑激酶（ｆｏｃａｌ ａｄｈｅｓｉｏｎ ｋｉｎａｓｅ，ＦＡＫ）的影响，明确层粘连蛋白（ｌａｍｉｎｉｎ，ＬＭ）反义ＲＮＡ对上述表达的调节作用。方法 采用免疫抑迹技术检测各蛋白的表达水平，采用增强的化学发光技术显示结果。

Effect of laminin antisense RNA on the expression of five intracellular proteins stimulated by transforming growth factor beta(1)]

OBJECTIVE: To investigate the effect of laminin antisense RNA on laminin antisense RNA on the expression of E-cadherin, alpha-, beta-, gamma-catenins and focal adhesion kinase(FAK) stimulated by transforming growth factor beta(1)(TGFbeta(1)). METHODS: Western blot technique was used to study their expression at protein level with ECL reagent. RESULTS: The expression of alpha- and gamma-catenins was up regulated by TGF beta(1) without the interference of laminin antisense RNA. The up regulation of E-cadherin stimulated by TGFbeta(1) was inhibited in the presence of laminin antisense RNA. No change was found in the expression of FAK when stimulated by TGFbeta(1), alone, but its expression was down regulated with the help of laminin antisense RNA. CONCLUSION: The inhibition of TGFbeta (1) on the malignancy of colon cancer cell may partially be mediated by the up regulation of E-cadherin and catenins. The effect of TGFbeta(1) on the expression of E-cadherin and FAK may be the secondary results of the change in laminin expression.