| 人lrg真核表达载体的构建和初步分析 |
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| 引用本文： | 杜可军,柴玉波,常文辉,段小红,侯理朝,陈南春,林树新,陈苏民. 人lrg真核表达载体的构建和初步分析[J]. 牙体牙髓牙周病学杂志, 2004, 14(4): 179-182 |
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| 作者姓名： | 杜可军 柴玉波 常文辉 段小红 侯理朝 陈南春 林树新 陈苏民 |
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| 作者单位： | 第四军医大学生物化学和分子生物学教研室,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目 (3 0 170 3 61) |
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| 摘 要： | 目的：在真核细胞中表达人lrg基因。方法：构建野生型人lrg的真核表达载体，体外转染肝癌细胞系HepG2，并进行稳定筛选。用Western Blot、SABC-FITC进行人lrg基因表达的鉴定。结果：成功构建了人lrg的真核表达载体pcDNA3．1( )／hlrg，Western Blot和SABC-FITC等实验表明该基因在HepG2中进行了表达。结论：在HepG2中稳定表达了pcDNA3．1( )／hlrg，为深入探讨人lrg基因的功能奠定了基础。
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| 关 键 词： | 人lrg基因 真核表达载体构建 HepG2细胞 |
| 文章编号： | 1005-2593(2004)04-0179-04 |
| 修稿时间： | 2003-12-31 |
Construction of human lrg eukaryotic expression vector and primary assay |
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| DU Ke-jun,CHAI Yu-bo,CHANG Wen-hui,et al. Construction of human lrg eukaryotic expression vector and primary assay[J]. Chinese Journal of Conservative Dentistry, 2004, 14(4): 179-182 |
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| Authors: | DU Ke-jun CHAI Yu-bo CHANG Wen-hui et al |
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| Abstract: | AIM:To expression human lrg in eukaryotic cells.METHODS:Eukaryotic expressive vector of human lrg was constructed, and then the plasmid was transfected into HepG2 cell line using lipid transfection reagent.Expression of lrg was detected by Western Blot and SABC-FITC.RESULTS:Construction of eukaryotic expression vector pcDNA3.1(+)/ hlrg ,and expression of hlrg in HepG2 were confirmed by Western Blot and SABC-FITC.CONCLUSION: Expression of pcDNA3.1(+)/ hlrg in HepG2 may throw light on the further study of the function of human lrg . |
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| Keywords: | human lrg gene construction of eukaryotic expression vector HepG2 cell line |
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