BCL-2及P53mt蛋白筛检砷中毒致皮肤癌的价值

目的：探讨BCL－2及P53mt蛋白筛检砷中毒致皮肤癌的价值。方法：BCL－2及P53mt蛋白折检测用免疫组化染色法；两指标阳性率的分析用X^2检验；分析评价筛价值的有关指标。结果：无论是P53mt还是BCL－2，癌变组的阳性率均高于非癌变组，差异均有统计学意义（P53mt，P＜0．01）；BCL－2，P＜0．05），其中OR值分别为14．22（2．93－68．97）和8．76（1．07－71．51）。P53mt的约登指数为0．529，明显高于BCL－2的0．284。串联试验可提高筛检价值。并联试验则降低了筛检价值。结论：P53mt与BCL－2均可用于筛检砷中毒所致皮肤癌，将两指标串联可提高筛检价值。

Value of BCL-2 and P53mt proteins in screening skin carcinoma caused by arseniasis

OBJECTIVE: To discuss the value of P53mt and BCL-2 proteins in screening skin carcinoma due to arseniasis. METHODS: P53mt and BCL-2 proteins were detected by immunohistochemical staining. chi(2) test was used to analyze the difference of positive rate between two groups. Screening value of the two biomarkers was also evaluated through the analysis of relative indexes. RESULTS: Positive percentages of P53mt and BCL-2 in carcinoma group were 88.89% and 94.44% respectively, both were higher than those of 36.0% and 66.0% in non-carcinoma group (for P53mt, P < 0.01; for BCL-2, P < 0.05). ORs of P53mt and BCL-2 were 14.22 (2.93 - 68.97) and 8.76 (1.07 - 71.51), respectively. Youden's Index and specificity of P53mt were 0.529 and 64.0%, which were much higher than those of BCL-2. Serial tests improved the value of screening with Youden's Index 0.569, but parallel test lowered it to 0.244. CONCLUSIONS: P53mt and BCL-2 were practical biomarkers to screen skin carcinoma due to arseniasis, and the former was better than the latter. The value of screening can be improved by a series of tests.