| Preparation and Preservation of Hypoxia UW Solution |
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| 引用本文： | 万赤丹,王春友,刘涛,程锐,杨智勇. Preparation and Preservation of Hypoxia UW Solution[J]. 华中科技大学学报(医学英德文版), 2007, 27(5): 554-556. DOI: 10.1007/s11596-007-0520-4 |
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| 作者姓名： | 万赤丹 王春友 刘涛 程锐 杨智勇 |
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| 作者单位： | Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Department of General Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China，Wuhan 430022 China |
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| 摘 要： | In order to explore the method to prepare hypoxia UW solution and the stability and preservation of hypoxia UW solution, UW solution was purged by argon or air for 15 min or 60 at a flow rate of 0.8 or 2 L/min, and the oxygen partial pressure of UW solution was detected. The hypoxia UW solution was exposed to the air or sealed up to preserve by using different methods, and the changes of oxygen partial pressure was tested. The results showed that oxygen partial pressure of 50 mL UW solution, purged by argon for 15 min at a flow rate of 2 L/min, was declined from 242±6 mmHg to 83±10 mmHg. After exposure to the air, oxygen partial pressure of hypoxia UW solution was gradually increased to 160±7 mmHg at 48 h. After sealed up by the centrifuge tube and plastic bad filled with argon, oxygen partial pressure of hypoxia UW solution was stable, about 88±13 mmHg at 72 h. It was concluded that oxygen of UW solution could be purged by argon efficiently. Sealed up by the centrifuge tube and plastic bag filled with argon, oxygen partial pressure of UW solution could be stabilized.
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| 关 键 词： | 器官保存溶液 氧分压力 组织缺氧 细胞水肿 |
| 收稿时间： | 2006-11-12 |
Preparation and preservation of hypoxia UW solution |
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| Wan Chidang,Wang Chunyou,Liu Tao,Cheng Rui,Yang Zhiyong. Preparation and preservation of hypoxia UW solution[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2007, 27(5): 554-556. DOI: 10.1007/s11596-007-0520-4 |
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| Authors: | Wan Chidang Wang Chunyou Liu Tao Cheng Rui Yang Zhiyong |
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| Affiliation: | Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary In order to explore the method to prepare hypoxia UW solution and the stability and preservation of hypoxia UW solution, UW solution was purged by argon or air for 15 min or 60 at a flow rate of 0.8 or 2 L/min, and the oxygen partial pressure of UW solution was detected. The hypoxia UW solution was exposed to the air or sealed up to preserve by using different methods, and the changes of oxygen partial pressure was tested. The results showed that oxygen partial presure of 50 mL UW solution, purged by argon for 15 min at a flow rate of 2 L/min, was declined from 242±6 mmHg to 83±10 mmHg. After exposure to the air, oxygen partial pressure of hypoxia UW solution was gradually increased to 160±7 mmHg at 48 h. After sealed up by the centrifuge tube and plastic bad filled with argon, oxygen partial pressure of hypoxia UW solution was stable, about 88±13 mmHg at 72 h. It was concluded that oxygen of UW solution could be purged by argon efficiently. Sealed up by the centrifuge tube and plastic bag filled with argon, oxygen partial pressure of UW solution could be stabilized. |
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| Keywords: | organ preservation solution oxygen partial pressure hypoxia |
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