心肌缺血预适应对缩小心肌梗塞范围的临床研究

用培养的兔腹腔巨噬细胞条件培养基作为趋化因子的来源，用改良的Ｂｏｙｄｅｎ小室微孔滤膜法进行单核细胞趋化试验，观察了兔腹腔巨噬细胞条件培养基对单核细胞的趋化作用和抗单核细胞趋化蛋白－１（ｍｏｎｏｃｙｔｅｃｈｅｍｏａｔｔｒａｃｔａｎｔｐｒｏｔｅｉｎ－１，ＭＣＰ－１）抗体对单核细胞迁移的影响。同时用Ｎｏｒｔｈｅｒｎｂｌｏｔ分析方法检测了ＭＣＰ－１ｍＲＮＡ在该巨噬细胞的表达。结果显示，该条件培养基对单核细胞有明显的趋化作用，并被抗ＭＣＰ－１抗体所抑制。同时该巨噬细胞也能表达ＭＣＰ－１ｍＲＮＡ。这提示，巨噬细胞能分泌ＭＣＰ－１，并招引单核细胞迁入内皮下间隙，从而在动脉粥样硬化的发病过程中发挥重要的作用。

Production of Monocyte Chemoattractant Protein-1 by in Rabbits Peritoneal Exudate Macrophages

The monocyte migration induced by the media conditioned by cultured rabbit peritoneal exudate macrophages was assayed by micropore filter method using modified Boyden chamber.Influence of anti-MCP-1 antibody on monocyte migration was observed as well.Meanwhile,we examined the expression of MCP-1 mRNA in the macrophages by Northern blot analysis. Results Cultured rabbit peritoneal exudate macrophages produced a factor that was chemotactic for monocytes,and the activity due to chemotaxis and not chemokinesis,the chemotactic activity was markedly inhibited by anti-MCP-1 antibody. MCP-1 mRNA was expressed by the macrophages.Conclusions Macrophages can produce MCP-1 and play an important role in atherogenesis through attracting monocytes to migrate into subendothelial space.