限食及限水状态下肺、肠组织血管活性肠肽、胆囊收缩素、P物质表达差异的实验研究

目的：比较限食、限水及生理状态下,肺与空肠、回肠、结肠组织上的血管活性肠肽（VIP）、胆囊收缩素（CCK）、P物质（SP）的表达差异,由＂肠病及肺＂探讨肺与大肠的关系。方法：27只SD雌性大鼠,分成限食组、限水组、生理组,每组9只。限食组给予平均进食量的1/2;限水组给予每天饮水量的1/2;生理组正常饮食,饲养7 d。用放射免疫分析法检测各组大鼠肺、空肠、回肠、结肠组织上的VIP、CCK、SP含量。结果：限食组大鼠肺与空肠、回肠、结肠的CCK表达比较,差异无统计学意义（P〉0.05）;与空肠的VIP、SP表达比较,差异有统计学意义（P〈0.05）;与回肠、结肠比较,无显著差异;限水组大鼠肺与空肠的VIP、SP比较,表达有显著差异（P〈0.05）;与回肠、结肠表达比较,差异有统计学意义（P〉0.05）;肺与空肠、回肠、结肠的CCK表达比较,差异无统计学意义（P〉0.05）;生理组大鼠肺与空肠的VIP、CCK、SP表达比较,差异有统计学意义（P〈0.05）;与回肠、结肠表达比较,差异无统计学意义（P〉0.05）。结论：无论在限食、限水或者是在生理状态下,肺、结肠和回肠这三者之间的关系都最为密切。同时,VIP、CCK及SP这三种脑肠肽物质是肺、肠之间的物质基础,VIP、SP相较于CCK,更有可能是肺与大肠之间关系密切的物质基础。

The Expression Difference of VIP,CCK and SP in Lung and Bowels in Limited-food and Limited-water States

Objective：To compare the expression difference of VIP,CCK and SP in lung,jejunum,ileum and colon in limited-food,limited-water and physiological states.Then discuss the relationship between lung and large intestine.Methods：30 female health SD rats were divided into limited-food group,limited-water group and physiological groups.The limited-food group was given half of the food.The limited-water group was given half of the water.The physiological group was euphagia.Analyze the VIP,CCK and SP content of lung,colon,jejunum and ileum with radio immunoassay.Results：The CCK expression of lung,jejunum,ileum and colon has no significant differences.VIP and the SP expression compared with jejunal has significant differences but no significant differences compared with colon and ileum in limited-food group The VIP,CCK and SP expression in lung and jejunal has significant differences but no significant differences compared with colon and ileumin in limited-water group.VIP,CCK and SP content of lung,jejunum has significant differences.but no significant differences compared with colon and ileum.Conclusion：The lung,ileum and colon have the most closely relationship under the three states.VIP,CCK and SP these three braingut petide are the material base between the lung and bowels.VIP and SP are more possible.