河南产丹参水溶性提取物提取工艺研究

目的：探讨丹参水溶性提取物的最佳提取工艺。方法：以酸水为溶剂，加热回流法提取丹参水溶性提取物，采用正交试验设计考察时间、温度、pH值、提取溶剂体积等4因素，对丹参水溶性成分的影响，筛选出最佳的提取工艺；以丹酚酸B为标准品，采用HPLC法测定含量。结果：丹参水溶性提取物以酸水做溶剂，最佳提取工艺为pH值为3的酸水，8倍量，温度100℃，加热回流4h。结论：本提取工艺所得提取物丹酚酸B含量较高，简单，经济，可行性强，适合工业大批量生产。

Study on the Extraction Process of the Water.Soluble Extracts of Salvia Miltiorrhiza Produced in Henan Province

Objective：To study the optimum extraction process of water-soluble extracts of Salvia mihiorrhiza. Methods：Acidic water was used as the solvent and the heating reflux method was adopted for the extraction of water-soluble extracts of Salvia mihiorrhiza. The orthogonal experimental design was applied to inspect the influence of four factors such as time, temperature, volume and extraction solvent volume on the water-soluble extracts of Salvia miltiorrhiza in order to select the optimum extraction process. With salvianolic acid B as the standard,the content was determined by HPLC. Results：The water-soluble extracts of Salvia mihiorrhiza used acidic water as solvent. The optimum extraction process was as follows, acidic water with PH of 3, eight times of the amounts, the temperature of 100 ℃ and the heating reflux of 4 h. Conclusion：This extraction process can extract higher contens of salvianolic acid B,which is simple, economic, feasible and suitable for industrial mass production.