| 反相高效液相色谱法测定盾叶薯蓣中薯蓣皂苷的含量 |
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| 引用本文： | 史君星,左春芳,谢彩侠.反相高效液相色谱法测定盾叶薯蓣中薯蓣皂苷的含量[J].河南中医药学刊,2012(8):983-984. |
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| 作者姓名： | 史君星 左春芳 谢彩侠 |
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| 作者单位： | [1]安阳市人民医院,河南安阳455000 [2]河南中医学院,河南郑州450046 |
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| 基金项目： | 河南省高等学校青年骨干教师资助计划（编号：2010GGJS-134）,河南省教育厅自然科学研究项目资助（编号：2011A360021） |
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| 摘 要： | 目的：探讨盾叶薯蓣中薯蓣皂苷含量测定的方法。方法：采用反相高效液相色谱法。色谱柱为DIKMAC18色谱柱（250mm×4．6mm，5μm），流动相为乙腈-水（52：48），流速为1．0mL·min^-1，检测波长为203．0nm，柱温为30℃。结果：薯蓣皂苷在0．40-4．01μg范围内线性关系良好，相关系数r=0．9997（n=7）。平均回收率为101．65％，RSD为2．29％（n=6）。结论：该方法简便、准确、快速、灵敏、重现性好，可以作为盾叶著蓣的质量控制方法。
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| 关 键 词： | 盾叶薯蓣 反相高效液相色谱法 薯蓣皂苷 |
RP.HPLC Method for the Content Determination of Dioscin in Dioscorea Zingiberensis Wright |
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| Shi Junxing,Zuo Chunfang,Xie Caixia.RP.HPLC Method for the Content Determination of Dioscin in Dioscorea Zingiberensis Wright[J].Journal of Henan college of Traditional Chinese Medicine,2012(8):983-984. |
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| Authors: | Shi Junxing Zuo Chunfang Xie Caixia |
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| Institution: | Anyang People＇s Hospital, Anyang, Henan, China 455000 Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China 450046 |
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| Abstract: | Objective：To explore a method for the determination of the conten of dioscin in Dioscorea zingiberensis wright. Methods：RP- HPLC was used with DIKMA C18 chromatography eolumn （250 mm· 4.6 ram,5 μm） as the column and acetonitrilewater（ 52：48 ） as the mobile phase. The flow rate of the mobile phase was 1.0 mL · min^- l and the detection wavelength was 203.0 nm. The column temperature was 30 ℃. Results： The linear range for dioscin was 0.40 - 4.01 μg and the correlation coefficient was 0.999 7 （ n = 7 ）. The average recovery rate was 101.65 % and RSD was 2.29% （n = 6 ）. Conclusion：This method is simple, accurate, rapid, sensitive, reproducible,which can be regarded as the quality control method for Dioscorea zingiberensis wright. |
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| Keywords: | Dioscorea zingiberensis wright high performance liquid chromatography（HPLC） dioscin |
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