实验性脾虚证大鼠回肠P物质和血管活性肠肽含量的变化

应用放射免疫分析 (RIA)方法 ,对实验性脾虚证大鼠回肠始段P物质 (SP)、血管活性肠肽 (VIP)进行测定 ,并与对照组、自然恢复组和治疗组进行比较。发现脾虚组SP含量显著升高 (P <0 .0 0 5 ) ,VIP含量显著降低 (P <0 .0 5 ) ;自然恢复组与脾虚组比较 ,SP含量明显恢复 (P <0 .0 0 1 ) ,但未至正常 ,与对照组比较仍有显著差异 (P <0 .0 1 ) ,VIP含量无明显改变。加味四君子汤治疗组与脾虚组比较 ,SP含量降低 (P <0 .0 0 1 )至正常 (P >0 .1 0 ) ,VIP含量有较强的恢复趋势 ,但与脾虚组、自然恢复组均无显著差异。结果提示 :脾虚证大鼠回肠始段SP、VIP含量改变可能与脾虚证胃肠功能紊乱有关 ,加味四君子汤对实验性脾虚证大鼠回肠始段胃肠肽的分泌异常有一定的调理作用。

Changes of SP and VIP in Ileum of Experimental Spleen Deficiency Rats

Substance P (SP) and vasoactive intestinal peptide (VIP) levels in all layers of initial part of ileum in rats were measured by radioimmunoassays (RIA) in order to explore the mechanism of spleen deficiency (SD).SP levels in ileum of experimental SD rats were significantly higher than that of the control groups( P <0.005)while the VIP levels in ileum of SD rats were significantly lower than the control groups( P <0.05). In spontaneous recovery cases, SP levels recovered significantly(compared with the SD rats, P <0.001)and did not drop down to normal until the VIP levels recovered significantly. After the SD rats were treated with Chinese herbs (Sijunzi Tang), SP was improved (compared with the SD cases, P <0.001)and returned to normal(compared with the controls, P >0.10). So the results showed that changes of SP and VIP levels in initial part of ileum of SD rats may be closely related to the gastrointestinal motility disorders presented in SD and the Chinese herbs (Sijunzi Tang) currently used has a partially therapeutic effect.