甘珀酸对兔脑血管痉挛时缝隙连接蛋白43磷酸化表达的影响

目的 探讨甘珀酸对兔实验性蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)时缝隙连接蛋白43(Cx43)磷酸化表达的影响.方法 建立兔二次SAH模型,脑池及静脉分别给予甘珀酸,脑血管造影及光镜观察分析基底动脉直径及形态学变化并应用Western blot检测基底动脉Cx43蛋白磷酸化表达的变化.结果 SAH组与正常组相比,脑血管造影及光镜观察结果 证实基底动脉痉挛明显;痉挛动脉肇磷酸化的Cx43(P-Cx43)蛋白表达显著升高,但去磷酸化的Cx43(NP-Cx43)蛋白表达显著减少.甘珀酸脑池处理组及静脉处理组与SAH组相比,脑血管造影及光镜观察结果 证实基底动脉痉挛显著减轻;痉挛动脉壁P-Cx43蛋白表达显著减少,但NP-Cx43蛋白表达显著升高.结论 SAH后,Cx43蛋白磷酸化表达发生变化,脑池或静脉给予甘珀酸能明显缓解SAH后CVS,其作用机制可能与基底动脉Cx43蛋白磷酸化表达变化有关.

Expression of connexin 43 phosphorylation on cerebral vasospasm and effects of carbenoxolone on its expression in rabbits

Objective The study was designed to investigate the expression of Connexin43 ( Cx43) phosphorylation in the basilar arteries on cerebral vasospasm after experimental subarachnoid hemorrhage(SAH) and the effects of carbenoxolone(CBX) on CVS and Cx43 phosphorylation expression in rabbits. Method The double - hemorrhage model of SAH with injecting into the cistema magna was used. CBX was given intracistemally or intravenously. Angiography and hematoxylin and eosin staining were performed to observe the diameter and for morphological study of the basilar artery. Western blotting was used to analyze the phosphorylation expression of Cx43 protein in basilar artery. Results After SAH, arterial narrowing and morphological changes in vascular structure were significantly more than that in normal group. CBX inhibited these changes; compared to that in normal group, phosphorylated Cx43 was up - regulated, while dephosphorylated Cx43 was down - regulated in the basilar artery vessel wall after SAH. CBX successfully reversed these alterations of Cx43 phosphorylation. Conclusions The expression changes of Cx43 phosphorylation plays an important role in the pathogenesis of CVS. CBX reduces cerebral vasospasm after SAH by modulating the alterations of Cx43 phosphorylation.