兔VX2肝癌模型制作及综合影像评价

目的　介绍应用VX2 细胞株制作兔移植性肝癌模型 ,并用CT、MR等影像学检测手段筛选实验动物 ,评价瘤灶生长情况变化。方法　实验动物为新西兰大白兔 (60只 ) ,瘤株采用动物自身接种传代。VX2 瘤块组织接种于肝左叶 ,40只于接种后 3～ 4周行螺旋CT平扫及增强动态双期扫描、MR扫描 ,并行DSA造影。 2 0只于接种后 5周行上述检查。结果　 5 1只 (85 % )接种成功。CT平扫瘤体为低 /等密度灶 ,动脉期明显增强 ,门脉期呈低密度。MR平扫呈稍长T1、稍长T2 信号。肝动脉造影瘤体富血供 ,由肝动脉供血。注入碘油后可见瘤区碘油沉积。种植后 5周瘤体均有不同程度坏死。结论　移植性兔VX2 肝癌类似人类原发性肝癌 ,是介入治疗实验研究较为理想的动物模型。综合影像评价有利于实验动物的筛选

Making Rabbit Model with VX2 Liver Tumor and Multiple Imaging Evaluation

Objective To introduce an animal model of implanted VX 2 with liver tumor in rabbits,and to evaluate the growth of the tumor,select the suitable experimental animal model by CT and MR.Methods Sixty New Zealand white rabbits were implanted with VX 2 tumor in the left lobe of the liver.CT scanning was performed in forty rabbits after implantation 3~4 weeks,including plain,early aterial phase and portal phase images.The MR scanning and DSA were performed also.And so did in twenty animals who had been implanted for 5 weeks.Results Fifty-one(85%) rabbits were successfully implanted with VX 2 tumor in the liver.The tumors demonstrated itself as hypodensity or isodensity on plain CT scans,marked enhanced or ringe-enhanced on early arterial phase images,hypodensity on portal phase images.On MR scans the tumors were seen as hypointensity on T 1WI,and hyperintensity on T 2WI.Hepatic angiography showed that the tumors were hypervascular,and hepatic artery was the main blood-supplying vessel.Hyperdensity had been seen in the area of tumor after the lipidol was injected.Necrosis was found in the area of all rabbits' liver tumors at 5 weeks after implantation.Conclusion The rabbits model with VX 2 liver tumor is similar to the primary hepatic carcinoma of human beings,it is more suitable for interventional experimental study.Multiple imaging evaluation is very useful in selecting experimental animal.