Treatment with cystamine reduces apoptosis in liver from NZB/W F1 mice |
| |
Authors: | Tzang Bor-Show Chiang Szu-Yi Lai Wen-Xian Tsai Chun-Chou Wu Jen-Huang Hsu Tsai-Ching |
| |
Affiliation: | Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, ROC. bstzang@csmu.edu.tw |
| |
Abstract: | Increased population with hepatic diseases and apoptosis were found in patients with SLE and implicated in the pathogenesis of SLE. Since cystamine has been demonstrated to be beneficial to NZB/W F1 mice in our previous report, this study intends to investigate the effects of cystamine in liver from NZB/W F1 mice. Decreased apoptosis was detected in liver from NZB/W F1 mice given cystamine as compared to those given PBS by TUNEL and caspase-3 activity assay. Fas-dependent apoptotic proteins including Fas, cleaved caspase-8 and tBid were reduced in liver from NZB/W F1 mice given cystamine as compared to those given PBS. Additionally, the mitochondria-dependent apoptotic proteins including cytochrome c and Apaf-1 were reduced in liver from NZB/W F1 mice given cystamine as compared to those given PBS. Moreover, increased BCL-2 protein was observed in liver from both mice. Notably, increased NF-kappaB protein was detected in liver from NZB/W F1 mice given cystamine as compared to those given PBS. These experimental results suggest the effect of cystamine in reducing apoptosis in liver from NZB/W F1 mice through Fas-dependent and mitochondrial-dependent pathways. The phosphorylation of NF-kappaB (p65) could be a possible mechanism involving anti-apoptotic effects of cystamine in liver from NZB/W F1 mice. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|