| 生物活性因子对牛卵母细胞体外成熟及胚胎体外发育的影响 |
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| 引用本文： | 施巧婷,黄俊成,杨梅,王立虎,李辉,玛依拉,郭志勤. 生物活性因子对牛卵母细胞体外成熟及胚胎体外发育的影响[J]. 国外畜牧学(草食家畜), 2001, 0(Z2) |
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| 作者姓名： | 施巧婷 黄俊成 杨梅 王立虎 李辉 玛依拉 郭志勤 |
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| 作者单位： | 新疆畜牧科学院农业部畜牧兽医生物技术重点开放实验室!新疆乌鲁木齐830000，新疆畜牧科学院农业部畜牧兽医生物技术重点开放实验室!新疆乌鲁木齐830000，新疆畜牧科学院农业部畜牧兽医生物技术重点开放实验室!新疆乌鲁木齐830000，新疆畜牧科学院农业部畜牧兽医生物技术重点开放 |
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| 摘 要： | 免疫活性肽 (ICP)和表皮生长因子 (EGF)是两种生物活性因子 ,ICP对牛卵母细胞体外成熟及胚胎体外培养的影响还未见报道。本实验将ICP ( 1 0、1 0 0、50 0ng/ml)和EGF ( 2 .5、2 5、50ng/ml)加入成熟液和培养液中 ,对照组中不含生物活性因子。结果表明ICP在浓度为 50 0ng/ml时对卵母细胞成熟有抑制作用 ,对胚胎发育无明显作用 ;EGF( 50ng/ml)对牛卵母细胞成熟和胚胎发育均有促进作用
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| 关 键 词： | 牛 卵母细胞 胚胎 免疫活性肽 表皮生长因子 |
Effect of bio-active factor on in vitro maturation of bovine oocytes and culture of embryos |
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| SHI Qiao-ting,HUANG Jun-cheng,YANG Mei,WANG Li-hu LI Hui,MA Yila,GUO Zhi-qin. Effect of bio-active factor on in vitro maturation of bovine oocytes and culture of embryos[J]. Grass-Feeding Livestock, 2001, 0(Z2) |
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| Authors: | SHI Qiao-ting HUANG Jun-cheng YANG Mei WANG Li-hu LI Hui MA Yila GUO Zhi-qin |
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| Abstract: | Immunocompentent peptide (ICP) and epidermal growth factor(EGF) are two bio- active factors, the effect of ICP on in vitro maturation of bovine oocytes and development of embryos not being reported. In this experiment, maturation medium contained 0,10,100,500ng/ml ICP or 0,2.5,25,50ug/ml EGF during IVM and IVC. The results showed that the group with 500ng/ml ICP has inhibiting effect on in vitro maturation of bovine oocytes. There was no significant difference between control group and the group with 10,100,500ng/ml ICP in blastocyst rate . EGF has positive effect on IVM and IVC of bovine oocytes development to blastocyst stage. |
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| Keywords: | Bovine Oocyte Embryo ICP EGF |
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