导数同步荧光法测定血清色氨酸

为了排除酷氨酸、苯丙氨酸对荧光法测定色氨酸的干扰，建立了色氨酸导数－同步荧光测定法。结果表明，当△λ为９０ｎｍ时，色氨酸的一个阶导数－同步荧光峰在２８５ｎｍ时不受酪氨酸、苯丙氨酸的干扰；色氨酸浓度在０～４００μｍｏｌ／Ｌ与导数－同步荧光值成线性，Ｙ＝０．２８７Ｘ±０．０９６，ｒ＝０．９９９；批内、批间ＣＶ分别为２．５７％、３．４２％；回收率９３．８％～９７．２％；血红蛋白含量在０．２５～５．０ｇ／

Determination of tryptophan in serum by derivative synchronous fluorescence method

To remove the interference from tyrosine and phenylalanine in the determination of tryptophan by fluorescence method,The author established the determination method of free tryptophan in serum by derivative synchronous fluorescence.The results showed,when was 90 nm,the peak of derivative synchronous fluorescence wasn't disturbed by tyrosine and phenylalanine at 285 nm. The linear rang is 0 to 400 mol/L(Y=0287X0096,r=0999).The intraand interassay CV are 257% and 342%,respectively.The analytical recovery is 938% to 972%. The determination wasn't disturbed by hemoglobin(02550 g/L)and bilirubin(4275342 mol/L).Reference value of the tryptophan in serum from 30 healthy individuals were 7398726( s ). The method is simple,rapid,and precise,so it is a reasonable method for detection of tryptophan in serum.