| 免疫胶体金标记电镜技术及其在病毒形态学研究中的应用 |
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| 引用本文： | 夏诗茂,凌静萍,陈维康,王丽华,梁沙,周静仪,迟捷,洪涛. 免疫胶体金标记电镜技术及其在病毒形态学研究中的应用[J]. 中国医学科学院学报, 1987, 0(1) |
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| 作者姓名： | 夏诗茂 凌静萍 陈维康 王丽华 梁沙 周静仪 迟捷 洪涛 |
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| 作者单位： | 中国预防医学科学院病毒学研究所，卫生部生物制品检定所，中国预防医学科学院病毒学研究所，煤炭部矽肺研究所，广西医学院，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所 北京，北京，北京，北京，北京 |
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| 摘 要： | 根据免疫胶体金电镜技术(IGEM),以不同方法制备了几种不同大小的胶体金颗粒,经SPA包被后对EHFV感染的Vero E-6细胞、轮状病毒感染的Ma 104细胞和CMV感染的人胚肺细胞分别进行免疫胶体金细胞表面标记、细胞穿透标记和超薄切片标记实验,并着重讨论了IGEM的优越性,适用范围和存在的问题。
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| 关 键 词： | 免疫胶体金电镜技术 病毒形态学 |
Immunocolloidal Gold Labelling Electron Microscopy and Its Application in Studying Morphology of Viruses |
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| Xia Shimao,Ling Jingping,Chen Weikang,Wang Lihua Liang Sha,Zhou Jingyi,Chi Jie and Hong Tao Institute of Virology,Chinese Academy for Proventive Medicine,Beijing, National Institute for Biological Products Control,Beijing,China. Immunocolloidal Gold Labelling Electron Microscopy and Its Application in Studying Morphology of Viruses[J]. Acta Academiae Medicinae Sinicae, 1987, 0(1) |
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| Authors: | Xia Shimao Ling Jingping Chen Weikang Wang Lihua Liang Sha Zhou Jingyi Chi Jie Hong Tao Institute of Virology Chinese Academy for Proventive Medicine Beijing National Institute for Biological Products Control Beijing China |
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| Affiliation: | Xia Shimao,Ling Jingping,Chen Weikang,Wang Lihua Liang Sha,Zhou Jingyi,Chi Jie and Hong Tao Institute of Virology,Chinese Academy for Proventive Medicine,Beijing, National Institute for Biological Products Control,Beijing,China, Institute of Silicosis,Chinese Ministry of Coal, Cuang Xi Medical College, |
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| Abstract: | Immunocolloidal gold electron microscopy (IGEM) hive been established and modified in our laboratory according to the data published. Several sizes of colloidal gold particles (GP) were prepared respectively with different methods and further coated with SPA.Colloidal gold labelling experiments of VeroE-6 cells infected with epidemic hemorrhagic fever virus (EHFV) ,Maj104 cells infected with rotavirus and human embryo lung cells infected with cytomegalovirus(CMV) were carried out by means of the preembedding method(including label both on the surface and in the inside of cells)and the postembedding method,and the results were ideal.In this paper, IGEM was emphatically discussed on its superiority, fields of utilization and some problems appearing in the study. |
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| Keywords: | immunocolloidal gold electron microscopy morphology of virus |
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