腺病毒介导的TEM8抑制小鼠在体胶质瘤模型的血管生成作用

目的探讨基于TEM8的腺病毒(Ad-TEM8)对小鼠胶质瘤G422细胞系体内外模型的生长抑制及其抗血管的生成作用。方法连续皮下注射Ad-TEM8使其激活小鼠免疫系统并收集小鼠脾淋巴细胞,将该细胞与G422细胞体外共培养,检测G422细胞的生长抑制率,并应用ELISOT检测该免疫激活淋巴细胞在体外分泌IFN-γ的能力。另构建皮下荷G422昆明小鼠在体肿瘤模型,并连续皮下注射Ad-TEM8后检测肿瘤生长速度以及瘤内血管抑制效应。结果 Ad-TEM8能有效激发小鼠产生具有肿瘤生长抑制作用的毒性效应淋巴细胞,并分泌IFN-γ。Ad-TEM8皮下注射诱导的细胞毒性作用能有效抑制在体G422肿瘤生长,产生明显的血管生成抑制作用。结论 Ad-TEM8可诱导小鼠产生细胞毒性细胞、抑制在体和体外肿瘤细胞生长,其在体抑制肿瘤生长作用机制之一可能是抑制肿瘤内血管生成。TEM8可能成为抗胶质瘤血管生成治疗的新靶标。

Inhibitory effect of recombinant adenovirus expressing TEM8 gene against angiogenesis in mouse model of glioma

[Abstract] Objective To evaluate the antiangiogenesis effect of recombinant adenovirus carrying TEM8 (Ad-TEM8) on the growth of glioma G422 cells. Methods The Ad-TEM8 was prepared, and utilized to immunized Kungming mice bearing G422 cells. The potential of antiangiogenesis was observed. Results Ad-TEM8 could elicit the cytotoxicity T lymphocytes (CTLs) mediated immune response of Kungming mice to attack tumor endothelium cells, and reduce tumor growth. Conclusions Ad-TEM8 could induce antitumor immunity by disrupting tumor vasculature and may thus be used as an efficient strategy for glioma therapy.