| 曲古抑菌素A单用及联用伊马替尼对K562R细胞增殖和凋亡的影响 |
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| 引用本文： | Chen S,Liu Y,Zeng H,He Q,Zhao X,Chen F. 曲古抑菌素A单用及联用伊马替尼对K562R细胞增殖和凋亡的影响[J]. 中南大学学报(医学版), 2011, 36(8): 782-785. DOI: 10.3969/j.issn.1672-7347.2011.08.015 |
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| 作者姓名： | Chen S Liu Y Zeng H He Q Zhao X Chen F |
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| 作者单位： | 中南大学湘雅医院血液科, 长沙 410008 |
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| 摘 要： | 目的:探讨曲古抑菌素A( trichostatin A,TSA)对耐伊马替尼细胞株K562R的增殖和凋亡的影响.方法:采用3-(4,5-二甲基-2-基)-5（3-羧基甲基苯基）-2-（4-磺酸苯基）-2H-四氮唑细胞毒试验法观察细胞增殖抑制,细胞凋亡率采用流式细胞仪凋亡试剂盒检测.结果:TSA单用能抑制K562R细胞的...
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| 关 键 词： | 曲古抑菌素A 伊马替尼 耐药 增殖 凋亡 |
Effect of trichostatin alone and combination with imatinb on the proliferation and apoptosis of the K562R cells |
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| Chen Shuping,Liu Yi,Zeng Hui,He Qun,Zhao Xielan,Chen Fangping. Effect of trichostatin alone and combination with imatinb on the proliferation and apoptosis of the K562R cells[J]. Journal of Central South University. Medical sciences, 2011, 36(8): 782-785. DOI: 10.3969/j.issn.1672-7347.2011.08.015 |
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| Authors: | Chen Shuping Liu Yi Zeng Hui He Qun Zhao Xielan Chen Fangping |
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| Affiliation: | Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China |
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| Abstract: | Objective To explore the effect of trichostatin A (TSA) alone and combination with imatinib on the proliferation and apoptosis of K562R cells. Methods 3-(4,5-dimethylthiazol-2-yl) -5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay was used to observe the proliferation of K562R cells and apoptosis was analyzed by annexin-Ⅴ/propidium iodide(PI) staining.Results After exposure to TSA, the proliferation of K562R cells was inhibited, and the effect was in both time- and dose-dependent manner. The apoptosis was induced. Combined with imatinib, the effect of proliferation inhibition was better. Conclusion TSA combined with imatinib can inhibit the proliferation of K562R cells and induce its apopotosis. TSA may become a new drug for imatinib-resistant patients. |
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| Keywords: | trichostatin imatinb drug-resistant proliferation apoptosis |
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