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| 简易部分肝切除自体肝移植大鼠模型的建立 | |
| 引用本文： | 陈晚平,何鑫,李珂,刘初平,吴金术,叶启发. 简易部分肝切除自体肝移植大鼠模型的建立[J]. 湖南师范大学学报(医学版), 2011, 8(3): 45-47 |
| 作者姓名： | 陈晚平 何鑫 李珂 刘初平 吴金术 叶启发 |
| 作者单位： | 1. 湖南师范大学第一附属医院,湖南省人民医院肝胆医院,湖南长沙410007 2. 长沙市三医院临床药学室,湖南长沙,410007 3. 河南省人民医院肝胆外科,河南郑州,450008 4. 中南大学湘雅三医院,湖南长沙,410013 |
| 基金项目： | 长沙市科技计划资助项目(k0802084-31) |
| 摘 要： | 目的:建立一种简易的大鼠部分肝切除自体肝移植模型.方法:采用SD大鼠,在既往自体肝移植模型基础上进行简化和改进,摒弃传统的门、腔及颈内静脉置管转流方法,改进冷灌注方法,建立了研究大鼠部分肝切除自体余肝移植模型.结果:手术过程大大简化,成功率为96.7％.门静脉、下腔静脉置管时间不超过1min,无肝期为16～18(中位时... |
| 关 键 词： | 部分肝切除 自体肝移植 大鼠 动物模型 |
Construction and Identification of the shRNA lentiviral expression Vector for targeting mouse Dnd1 gene |
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| Chen Wanping,He Xin,Li Ke,Liu Chuping,Wu Jingshu,Ye Qifa. Construction and Identification of the shRNA lentiviral expression Vector for targeting mouse Dnd1 gene[J]. Journal of Hunan Normal University(Medical Science), 2011, 8(3): 45-47 | |
| Authors: | Chen Wanping He Xin Li Ke Liu Chuping Wu Jingshu Ye Qifa |
| Affiliation: | Li Shundong1,Yu Miao2,Zhang Yong2,Deng Lai3,Cheng Jie1,Peng Xiaoning2(1.The Third Hospital of Changsha,Changsha 410005,China,2.Medicine College,Hunan Normal University,Changsha 410006,3.The Third Xiangya Hospital of Center South University) |
| Abstract: | Objective To construct the shRNA lentiviral expression vector for targeting mouse Dnd1 gene.Methods Four pairs of sh RNAs that target at Dnd1 gene were designed.After annealed,the inserts were ligated into the linearized pGCSIL-GFP,and the vectors were identified by PCR and DNA sequencing.Results The target segments were cloned into pGCSIL-GFP lentivirus RNAi vector respectively,and the recombinant plasmids containing Dnd1 sh RNA was confirmed correct by PCR identification and DNA sequencing.Conclusion We s... |
| Keywords: | Dnd1 lentiviral vector RNAi |
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