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| HPLC法检测水产品中孔雀石绿、结晶紫及其关联化合物的残留 | |
| 引用本文： | 白丽丽,林正峰,戴华.HPLC法检测水产品中孔雀石绿、结晶紫及其关联化合物的残留[J].海南医学院学报,2011,17(9):1157-1159,1163. |
| 作者姓名： | 白丽丽 林正峰 戴华 |
| 作者单位： | 1. 海南医学院药学院,海南海口,571101 2. 海南出入境检验检疫局,海南海口,570311 3. 海南医学院公共卫生学院,海南海口,571101 |
| 基金项目： | 海南医学院科研基金资助学报项目(0020110302)~~ |
| 摘 要： | 目的:建立高效液相色谱法检测虾类水产品中孔雀石绿、结晶紫及其相关代谢物残留量的方法.方法:样品经乙腈提取后、浓缩、纯化,经Waters UPLC BEH C18柱（2.1mm×50 mm,粒径1.7 μm）分离,以0.5 mmol/L乙酸胺:乙腈=30∶70为流动相进行测定分析.结果:孔雀石绿、无色孔雀石绿的加标平均回... |
| 关 键 词： | 高效液相法 孔雀石绿 结晶紫 |
Determination of malachite green,crystal violet and their leuco-metabolites in aquatic product by HLPC |
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| BAI Li-li,LIN Zheng-feng,DAI Hua.Determination of malachite green,crystal violet and their leuco-metabolites in aquatic product by HLPC[J].Journal of Hainan Medical College,2011,17(9):1157-1159,1163. | |
| Authors: | BAI Li-li LIN Zheng-feng DAI Hua |
| Institution: | BAI Li-li1,LIN Zheng-feng2,DAI Hua3(1.Department of Pharmacy,Hainan Medical University,571101 Haikou China,2.Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou 570311,3.Department of Public Health,571101 Haikou China) |
| Abstract: | Objective： To establish a credible analytical method based on high performance liquid chromatography for the determination of malachite green（MG）,crystal violet and their leuco-metabolites in aquatic product.Methods： The MG,crystal violet and their leuco-metabolites were extracted by acetonitrile,then were concentrated and purified.Samples were isolated by Waters UPLC BEH C18 column（2.1 mm×50 mm,diameter 1.7 μm）and determined with 0.5 mmol/L ammonium acetate∶acetonitrile（30∶70） as mobile phase.Results： The mean recovery rate of MG and leucomalachite green were in the range of 90.3%-102.83% and 85.10%-98.60%,respectively.Values of RSD were 4.96%-6.88% and 6.24%-11.65%,respectively.Conclusions： The method is simple,sensitive,stable and suitable for the determination of trace malachite green and leucomalachite green green in aquatic product. |
| Keywords: | High performance liquid chromatography Malachite green Crystal violet |
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