Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography-mass spectrometry |
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Authors: | Mills G A Walker V Mughal H |
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Affiliation: | School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK. |
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Abstract: | Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography-mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen-polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories. |
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