肿瘤坏死因子相关凋亡诱导配体受体在肾癌中的分布及其意义 |
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引用本文: | 张志超,张智清,张强,张立国,曾革非,郭应禄. 肿瘤坏死因子相关凋亡诱导配体受体在肾癌中的分布及其意义[J]. 中华外科杂志, 2001, 39(5): 375-377 |
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作者姓名: | 张志超 张智清 张强 张立国 曾革非 郭应禄 |
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作者单位: | 1. 北京大学第一医院泌尿外科研究所 2. 中国预防医学科学院病毒所 |
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摘 要: | 目的:研究肿瘤坏死因子相关诱导凋亡配体(TRAIL)受体在肾癌组织中的分布及其意义。方法:采用RT-PCR及NorthernBlot的方法检测TRAIL受体在肾癌组织及正常肾组织,肾癌细胞系GRC-I和正常肾小管细胞系HK-2中的表达。结果:死亡受体DR4、DT4在肾癌组织及正常肾组织、肾癌细胞系GRC-I和正常肾小管细胞系HK-2中强表达;假受体DcR-1在正常肾组织和正常肾小管细胞系HK-2中强表达;假受体DcR-2未见表达。结论TRAIL基因在肾癌肿瘤细胞的凋亡机制中可能发挥重要的作用。
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关 键 词: | 肿瘤坏死因子 配体 肾肿瘤 细胞凋亡 TRAIL |
修稿时间: | 2000-10-23 |
Expression of tumor necrosis factor-related apoptosis-inducing ligand receptors in renal cell carcinoma |
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Abstract: | Objective To investigate the ex pression of tumor necrosis factor related apoptosis inducing ligand (TRAIL) r eceptors in renal cell carcinorma. Methods TRAIL receptors mRNA were tested through RT PCR and Northern Blot in renal cell carcinoma tissue and renal cell carcinoma cell line (GRC I). Results The expression of death receptor 4(DR4) and death recep tor 5(DR5) was certificated in renal cell carcinoma and normal renal cells. Deco y receptor 1(DcR1) expressed only in normal renal tissue and cell line. Decoy re ceptor 2(DcR2) was not detected in normal and tumor tissue and cell line. Conclusion TRAIL gene may play an important role in apoptosis of renal cell carcinoma. |
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Keywords: | Tumor necrosis factor Ligands Kindney neoplasms Apoptosis |
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