GC法测定茴拉西坦含量

目的建立气相色谱法测定茴拉西坦含量的方法。方法气相色谱内标法。色谱柱为DB-1弹性毛细管柱,FID检测器;汽化室温度为260℃,检测器温度300℃;柱温200℃,载气为氮气,流速为5.0mL·min-1,尾吹50 mL·min-1,分流比10∶1,以咖啡因为内标物。结果茴拉西坦在10～110μg·mL-1(r=0.9998)范围内呈良好线性关系,平均回收率为100.1%。结论本方法简便,快速,准确,可靠,可用于茴拉西坦的含量测定。

Determination of aniracetam by GC

Objective To establish a GC method for the determination of aniracetam. Methods The GC internal standard method was adopted. DB - 1 capillary column and FID detector were used. The injector temperature was 260 ℃,the detector tem- perature was 300 ℃ and the column temperature was 200 ℃. N2 was used as carrier gas at flow rate of 5.0 mL . min-1. The makeup flow was 50 mL . min 1 and the split ratio was 10： 1. Caffeine was used as internal standard. Results A good linearity of aniracetam was obtained within 10 - 110 μg . mL - l （ r = 0.999 8 ） and the average recovery was 100.1%. Conclusion The method was simple, rapid and accurate,and it can be used for the determination of aniracetam.