| RP—HPLC法测定护肝宁胶囊中大黄素的含量 |
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| 引用本文： | 罗轶,银胜高.RP—HPLC法测定护肝宁胶囊中大黄素的含量[J].广西中医学院学报,2008,11(3):72-74. |
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| 作者姓名： | 罗轶 银胜高 |
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| 作者单位： | 1. 广西壮族自治区食品药品检验所,广西,南宁,530023
2. 广西中医学院,广西,南宁,530001 |
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| 摘 要： | 目的]建立护肝宁胶囊中大黄素RP—HPLC的含量测定方法。方法]采用Kromasil—C18柱（5μm，4.6mm×250mm）；流动相为甲醇-0．1％磷酸溶液（85：15）；检测波长为289nm。结果]大黄素的进样量在0．1019～1．019μg范围内线性良好，r=0．9999；平均回收率为97．13％，RSD=1．36％（n=6）。结论]本法操作简便易行、灵敏度高、重现性好、准确可靠，可用于护肝宁胶囊的质量控制。
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| 关 键 词： | 虎杖 护肝宁胶囊 大黄素 RP—HPLC |
Determination of Emodin in huganning Capsules by RP-HLPC |
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| LUO Yi,YIN Sheng-gao.Determination of Emodin in huganning Capsules by RP-HLPC[J].Journal of Guangxi Traditional Chinese Medical University,2008,11(3):72-74. |
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| Authors: | LUO Yi YIN Sheng-gao |
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| Institution: | LUO Yi, YIN Sheng-gao (1. Guangxi Institute For Food And Drug Control, Nanning, 530021; 2. Guangxi Traditional Chinese Medical University, Nanning, 530001 ) |
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| Abstract: | Objective] To establish a special determination of Emodin in huganning capsules by RP- HLPC. Methods] Kromasil- C18 column （5μm,4.6mm×250mm）was choasen, The methanol- 0.1% phosphoric acid （gradient）was used as the mobile phase, and the detection wavelength was at 289nm. Results] For emodin ,the linear range was 0. 1019 - 1. 019μg and the average recovery was 97.08% with corresponding RSD= 1.37% （ n = 6）. Conclusion]The method is simple, sensitive, reproducible, accurate and can be used for the quality control of Chinese medicine Huganning capsules. |
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| Keywords: | RP-HPLC |
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