| 慢性不可预见性温和应激对大鼠行为及下丘脑胃泌素释放肽mRNA表达的影响 |
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| 引用本文： | 肖哲曼,姚丽华,郭青山,彭亮,杨灿,刘忠纯.慢性不可预见性温和应激对大鼠行为及下丘脑胃泌素释放肽mRNA表达的影响[J].国外医学：物理医学与康复学分册,2012,7(1). |
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| 作者姓名： | 肖哲曼 姚丽华 郭青山 彭亮 杨灿 刘忠纯 |
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| 作者单位： | 肖哲曼 (武汉大学人民医院神经内科,武汉430060) ； 姚丽华 (武汉大学人民医院神经精神疾病研究所,武汉430060) ； 郭青山 (武汉大学人民医院精神卫生中心,武汉,430060) ； 彭亮 (武汉大学人民医院精神卫生中心,武汉,430060) ； 杨灿 (武汉大学人民医院精神卫生中心,武汉,430060) ； 刘忠纯 (武汉大学人民医院神经精神疾病研究所,武汉430060) ； |
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| 基金项目： | 国家自然科学基金,湖北省自然科学基金,湖北省卫生厅青年人才基金 |
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| 摘 要： | 目的:研究慢性不可预见性温和应激(CUMS)对动物行为学和下丘脑胃泌素释放肽(GRP)mRNA表达的影响。方法:将14只大鼠随机分为抑郁组和对照组各7只,抑郁组采用CUMS制备抑郁模型。对2组大鼠进行行为学观察,使用荧光定量PCR检测GRP mRNA表达。结果:与对照组比较,抑郁组大鼠在应激后的糖水偏好和自主活动显著减低(P<0.001),GRP mR-NA表达升高(P<0.05)。结论:慢性应激后,出现抑郁样行为改变与GRP mRNA表达上调相关。
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| 关 键 词： | 慢性不可预见性温和应激 抑郁 行为学 胃泌素释放肽 |
Alterations of Behavior and Hypothalamic Gastrin-releasing Peptide mRNA Expression in Rats following Chronic Unpredictable Mild Stress |
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| Abstract: | Objective:To investigate the changes of behavior and expression of hypothalamic gastrin-releasing peptide(GRP) mRNA after chronic and unpredictable mild stress(CUMS).Methods:Fourteen rats were randomly divided into control group(n=7) and depression group(n=7).The rats in the depression group were subjected to CUMS for 21 consecutive days.The behavior of 2 groups were observed.The expression of GRP mRNA was measured by real-time quantitative PCR.Results:The autonomic activity and sugar consumption in the depression group were significantly lower than those in the control(P<0.001).The total distance,speed and rearing were significantly reduced in the stress group,revealed by the open field test(P<0.001).The expression of GRP mRNA in the stress group was significantly higher than that in the control group(P<0.05).Conclusion:The up-regulation of GRP mRNA probably plays a key in the behavior changes in rats exposed to stress. |
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| Keywords: | chronic unpredictable mild stress depression behavior gastrin-releasing peptide |
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